以分子标记辅助选择育成抗条纹叶枯病水稻新品种“武陵粳1号”  

A New Rice Cultivar "Wulingjing 1" with Resistance to Rice Stripe Virus Bred by Marker Assisted Selection

作　　者：潘学彪[1] 陈宗祥[1] 左示敏[1] 张亚芳[1] 吴旭江[1] 马宁[1] 江祺祥 阙金华 周春和 

机构地区：[1]扬州大学江苏省作物遗传生理重点实验室/植物功能基因组学教育部重点实验室,江苏扬州225009 [2]江苏省常州市武进区稻麦育种场,江苏常州213149 [3]江苏省种子管理站,江苏南京210036

出　　处：《作物学报》2009年第10期1851-1857,共7页Acta Agronomica Sinica

基　　金：国家科技支撑计划项目(2006BAD01A01-5);引进国际先进农业科学技术计划(948计划)重大项目(2006-G51)资助

摘　　要：条纹叶枯病是21世纪以来江苏省最重要的水稻病害之一。食味品质较优的主栽品种武育粳3号因高度感染该病而种植面积锐减。本研究以镇稻88为抗条纹叶枯病毒病基因Stvb-i的供体亲本,采用回交育种策略,改良武育粳3号的抗条纹叶枯病性能。在连续回交和自交过程中,以紧密连锁的双侧分子标记对Stvb-i进行"前景"选择,同时对后代与轮回亲本遗传背景的相似程度进行分子标记辅助"背景"选择,在短期内育成抗条纹叶枯病的"武育粳3号",命名为"武陵粳1号"。新品种保持了原品种的基本农艺性状、丰产性、稳产性和优异的食味品质,并大幅度提高了其条纹叶枯病抗性水平,在江苏省多点抗性鉴定试验中的平均病株率仅为4.4%,极显著低于原品种(53.2%)。Rice stripe virus disease is one of the most serious diseases in rice production area of Jiangsu province since 2002. A commercial rice cultivar with excellent food taste-quality, Wuyujing 3, was highly susceptible to the disease and was improved through the MAS strategy in a backcross project with “Zhendao 88” as donor parent of resistance gene Stvb-i. In each backcross generation, we selected the progenies with Stvb-i based on the closely flanked markers. In advanced backcross generations, the marker’s detection for genetic background of selected plants was performed, along with the estimate by the eye for the similarity of selected plants to recurrent parent in synthetic agronomic traits. As a result, a new cultivar, Wulingjing 1, was fleetingly bred in BC3F4 generation, whose resistance to the disease was significantly increased and main characters especially on excellent taste-quality were consistent with those of the recurrent parent Wuyujing 3. In resistance identification tests with multiple locations organized by Seed Management Station of Jiangsu Province, the average percent of affected plants of the new cultivar was only 4.4% and significantly lower than that of recurrent parent （53.2%）.

关 键 词：水稻条纹叶枯病 抗性基因供体 回交育种 轮回亲本 标记辅助选择 

分 类 号：S511.22[农业科学—作物学]