稀土元素对棒形杆菌生长和胞外酶活性的影响  

Effect of Rare Earth on the Growth and the Extracellular Enzyme Activity of Clavibacter michiganense subsp.michiganense

作　　者：章健[1] 刘庆都[1] 承河元[1] 

机构地区：[1]安徽农业大学植保系

出　　处：《安徽农业大学学报》1998年第1期81-84,共4页Journal of Anhui Agricultural University

摘　　要：为了解稀土元素对植物病原细菌的效应，研究了单一稀土氧化镧（Ｌａ２Ｏ３）对番茄细菌溃疡病棒形杆菌（Ｃｌａｖｉｂａｃｔｅｒｍｉｃｈｉｇａｎｅｎｓｅｓｕｂｓｐ．ｍｉｃｈｉｇａｎｅｎｓｅ，Ｃ３）的生长及胞外酶活性的影响。结果表明：稀土明显抑制Ｃ３的生长，且随处理浓度的提高，抑制作用加强。固体培养基上表现为Ｃ３菌落初生时间比对照延迟１２～４８ｈ，菌落直径减小１．３～２．９ｍｍ。液体培养基中表现为ＯＤ６２０值下降。稀土可明显提高Ｃ３的胞外蛋白酶、果胶酶和纤维素酶的活性。稀土浓度＞３５０ｍｇ／Ｌ时，Ｃ３停止生长。The effect of rare earth (RE) on the growth and extracellular enzyme activities of Clavibacter michiganense subsp.michiganense (C 3) were studied.At the RE concentrations of 50,100,150,200,250 and 300 mg/L the growth of C 3 was inhibited obviously both in solid and liquid culture and the inhibition increased with the rise of the RE concentration.The time of initial growth delayed,the diameter of colony decreased in solid medium and the biomass decreased in liquid culture.When RE concentration was greater than 300 mg/L,the growth of C 3 was inhibited completely.At 200 mg/L,RE evidently stimulated the activities of protease,cellulase and pectinase.

关 键 词：稀土 棒形杆菌 生长 胞外酶 活性 番茄病害 

分 类 号：S436.412[农业科学—农业昆虫与害虫防治]