BD针在重症急性胰腺炎急性肺损伤大鼠模型制备中的应用  

BD intravenous catheters used in inducing the model of acute lung injury with severe acute pancreatitis in rats

作　　者：鲍世韵 伍天崇 郭跃华 于会群[2] 何惠兰 

机构地区：[1]暨南大学第二临床医学院深圳市人民医院肝胆外科,深圳518020 [2]深圳市中医院,深圳518020 [3]深圳市盐田区医院,深圳351808

出　　处：《国际外科学杂志》2009年第10期657-660,F0003,共5页International Journal of Surgery

基　　金：深圳市科技计划项目（No.200802097）

摘　　要：目的探讨BD针在重症急性胰腺炎急性肺损伤研究中大鼠模型建立的改良应用。方法健康成年雄性SD大鼠40只，随机分为模型组（n=30）、假手术组（n=10），应用BD静脉留置针经十二指肠乳头逆行胰胆管注入4％牛磺胆酸（0.1mL/kg），建立重症急性胰腺炎急性肺损伤大鼠模型。模型组在建立后3h．6h、12h检测大鼠血淀粉酶、血气分析、腹水量、肺含水量，光镜下进行胰腺和肺组织病变程度评分。假手术组在术后12h检测上述相关指标。结果模型组较假手术组血淀粉酶、腹水量、肺含水率、PaCO2、胰腺和肺组织学评分明显升高（P〈0．05）。同时PaO2，氧合指数明显降低（P〈0．05）。结论应用BD静脉留置针能有效制备简便、易复制、稳定性好的重症急性胰腺炎急性肺损伤大鼠模型。Objective To investigate the improvement of BD intravenous catheters used in inducing the model of acute lung injury with severe acute pancreatitis in rats. Methods Total 40 healthy adult male SD rats were randomly assigned to 2 groups, SO group （ n = 10） , SAP group （ n = 30 ） , 4% tauroeholate （0. 1 ml/kg） was injected via retrograde biliopancreatic ducts by using BD intravenous catheters via duodenal papilla. The serum amylase, blood gas analysis, ascites volume, lung water content and the pathological score of pancreas and lung structure under light microscope were recorded when the SAP model were induced in 3 h ,6 h and 12 h. The relative data mentioned as above also detected in SO group after 12 h. Results The score of the SAP group was significantly higher than the SO group in blood amylase, ascites volume, lung water content, PaCO2 , pancreas and lung histology（ P 〈 0.05 ）. Meanwhile, PaO2, oxygenation index were decreased significantly （ P 〈 0. 05 ）. Conclusion It is efficient to establish simple, reproducible and stabile rat model of acute lung injury with severe acute pancreatitis by using BD intravenous catheters.

关 键 词：重症急性胰腺炎 急性肺损伤 动物模型 

分 类 号：R657.5[医药卫生—外科学] R-332[医药卫生—临床医学]