一种基于激光加工微型通道的光纤生物传感器  

A novel fiber optic biosensor based on microchannels fabricated Laser

作　　者：白忠臣[1,2] 姜崇杰[3] 秦水介[1] 李静[2] 张欣[1] 

机构地区：[1]贵州省光电子技术及应用重点实验室,贵州贵阳550025 [2]贵州大学理学院,贵州贵阳550025 [3]中国电子科技集团第四十九研究所,黑龙江哈尔滨150001

出　　处：《光电子．激光》2009年第5期605-608,共4页Journal of Optoelectronics·Laser

基　　金：国家自然科学基金资助项目(50735031);贵州省科技厅基金资助项目(黔科合J字[2007]2201);贵州大学校研究生创新基金资助项目(2007007)

摘　　要：提出一种新型结构的光纤生物传感器。利用的Nd:YAG激光(波长1064nm)在PMMA基片上加工制作合成的微米级的微型通道作为光纤生物传感器的样品检测微流路;利用化学腐蚀的方法在多模光纤上制作具有圆锥表面结构的分子识别探针和荧光接收探针。将分子识别探针、荧光接收探针和样品检测微流路组装成光纤生物传感器。在理论上,分析了荧光接收探针的圆锥形接收面的锥度角与接收到的荧光光线最小入射角之间的函数关系;在实验上,分析了分子识别探针附近的荧光强度与荧光接收探针接收到的荧光强度不同的原因。结果表明,该传感器在理论上和实验上是可行的;荧光接收探针的锥度角和分子识别探针与荧光接收探针的中心能否对准是影响接收到荧光多少的主要因素;同时,在检测溶液的浓度降低时,单个荧光分子的发光变得显著。A novel fiber optic biosensor is designed and theoretically analyzed in this paper. Nd：YAG laser （wavelength 1 064 nm） is used for processing complex micron channels of micro flow path of fiber optic biosensors on PMMA substrate. Chemical etching methods is used as fabricating the probe of molecular recognition and fluorescent receiving on muhimode optical fiber. Fiber optic biosensors are assembled by micro flow path and the probe of molecular recognition and fluorescent receiving. Relation between trap per angle and minimal fluorescent incident angle of the probe of fluorescent receiving is theoretically ana lyzed. Several reasons are why round fluorescence intensity of the probe of molecular recognition and flu orescent receiving is different are experimentally analyzed. The results indicate that fiber optic biosensors is theoretically and experimentally is feasible. Main factors of influencing fluorescent receiving depend on trapper angle of fluorescent receiving and the center alignment of the probe of molecular recognition and fluorescent receiving. At the same tome,when solution concentration is reduced,fluorescence of single molecule becomes remarkable.

关 键 词：光纤生物传感器 激光加工 微通道 锥度角 

分 类 号：O439[机械工程—光学工程] TN247[理学—光学]