全耐药鲍曼不动杆菌分子分型和分子流行病学调查  

Typing of Multi-drug Resistant Acinetobacter baumannii by PCR and Epidemiology Investigation

作　　者：欧阳萍[1] 黄晶[2] 雷连成[1] 尹立子[1] 林花[2] 吕爽[1] 韩文瑜[1] 

机构地区：[1]吉林大学畜牧兽医学院,长春130062 [2]吉林大学第一医院检验科,长春130021

出　　处：《生物技术通报》2009年第10期161-164,168,共5页Biotechnology Bulletin

摘　　要：重复片段引物PCR和随机扩增多态性DNA(RAPD)技术对临床分离全耐药不动杆菌分子分型,并进行流行病学调查。从ICU病房感染多重耐药不动杆菌患者的标本分离不动杆菌,碱裂解法提取全基因组,重复片段引物PCR(Rep-PCR)和随机引物扩增(RAPD),对8株临床分离的全耐药菌基因分型,并与生物学分型和质粒分型比较,调查医院流行全耐药菌的基因型。结果显示,8株分离菌经两对重复片段引物分型可分为6种和4种基因型,经随机引物分型为4种和3种基因型,经质粒分型可分为2种基因型,生物学分型归属为1种表型。PCR方法用于全耐药不动杆菌分子分型简便易行,重复性好,适合医院感染流行病学调查,本医院同一部门出现多种基因型,各科室间不存在交叉传染。It was to type multi-drug resistant bacteria Acinetobacter baumannii by Rep-PCR and RAPD-PCR then apply the results in the clinical strain identification and epidemiological study. Multi-drug resistant Acinetobacter baumannii was isolated from ICU ward and was determined by VITEK2 automatic microbial analyzer. The entire genome of Acinetobacter baumannii was extracted by alkaline lysis method and was amplificated by Rep-PCR and RAPD. DNA products clinically isolated from 8 strains of Acinetobacter baumannii was typed and compared with the antibiotic susceptibility spectrums and the results of conventional biotyping. With aid of Rep-PCR,the 8 strains of the bacterium were grouped into 6 and 4 and 3 types with 2 couples of repetitive pieces primer in the Pep PCR,4 and 3 types in RAPD,2 types in plasmid typing and one phenotype in biotyping. There was no cross-infection to exist in the hospital,that was proved by the multiple genotypes in a single department.

关 键 词：不动杆菌 聚合酶链反应 基因分型 流行病学调查 

分 类 号：R378[医药卫生—病原生物学] R181.3[医药卫生—基础医学]