太平洋多金属结核区深海沉积物细菌多样性分析  

Analysis of bacterial diversity in deep-sea sediments from Pacific polymetallic nodule province

作　　者：张文杰[1] 张心齐[1] 应意[1] 许学伟[1] 吴敏[1] 

机构地区：[1]浙江大学生命科学学院,浙江杭州310058

出　　处：《浙江大学学报（理学版）》2009年第5期578-585,共8页Journal of Zhejiang University（Science Edition）

基　　金：973项目子课题(耐辐射嗜盐古生菌中DNA修复机制研究);863子课题(核辐射污染环境微生物资源的开发利用);浙江省重大科技专项(富盐有机废水处理及回用技术)

摘　　要：从太平洋多金属结核区东区两个站点沉积物中直接提取环境总基因组,通过PCR及TA克隆分别构建了16S rRNA基因文库.经序列分析结果表明,2个文库共93个克隆分属13个类群,包括α变形菌纲、β变形菌纲、γ变形菌纲、δ变形菌纲、浮霉菌门、酸杆菌门、噬纤维菌-黄杆菌-拟杆菌群、硝化螺旋菌门、放线菌门、绿弯菌门、厚壁菌门、异常球菌-栖热菌门和OP11类群.其中,γ变形菌纲的细菌在2个站点沉积物中都是优势种群,变形菌纲、浮霉菌门、放线菌门、噬纤维菌-黄杆菌-拟杆菌群、硝化螺旋菌门是2个站点共有的细菌类群.DOTUR和LIBSHUFF数据分析结果表明,两个站点的沉积物均具有丰富的细菌多样性,并且物种组成具有显著性差异.The environmental total DNAs were directly extracted from two sediment samples from Pacific polymetallic nodule province （112. 950 3°W, 11. 884 9°N, water depth of 4 160 m; 145. 351 4°W, 8. 374 0°N, water depth of 5 257 m）. Two bacterial 16S rRNA gene libraries were generated by using PCR and TA cloning. Phylogenetie resuits indicated that 93 clones could be divided into 1. phylotypes, including Alphaproteobacteria, Gammaproteobacteria, Dehaproteobaeteria, Betaproteobacteria, Planctomycetes, Acidobaeteria, Cytophaga-Flavobacterium-Bacteroidetes （CFB）, Nitrospira, Actinobacteria, Chloroflex, Firmicutes, Deinococcus-Thermus, and candidate division OPll. 16S rDNA sequences from members of Gammaproteobacteria dominated two sediments samples. Proteobacteria （Alpha-, Gamma-, Beta-, Delta-）, Planctomycetes, Acidobacteria, CFB, Nitrospira appeared in two sediments samples. DOTUR and LIBSHUFF statistics of 16S rDNA gene sequences from the two libraries revealed major differences, indicating a high richness in the sediment bacterial communities and considerable variability in bacterial community composition among regions.

关 键 词：多金属结核区 深海沉积物 细菌多样性 16S RRNA基因 

分 类 号：Q933[生物学—微生物学]