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作 者:傅必谦[1] 陈卫[1] 高武[1] 张可心 袁虹 芮朝胜 李举怀[2]
机构地区:[1]首都师范大学生物系 [2]北京市农林科学院
出 处:《兽类学报》1998年第4期304-310,共7页Acta Theriologica Sinica
摘 要:中华姬鼠(Apodemusdraco)和大林姬鼠(Apodemuspeninsulae)是形态学上十分相似的两种鼠类。为了对两种姬鼠的分类提供生物化学方面的依据,采用聚丙烯酰胺凝胶等电聚焦电泳方法比较和分析了两种姬鼠的LDH同工酶、EST同工酶和SOD同工酶的差异。结果表明,两种姬鼠的LDH同工酶酶谱基本相似,而EST同工酶和SOD同工酶酶谱则存在明显的种间差异。根据EST同工酶A2带的有无和SOD同工酶主带等电点的差别,能将两种姬鼠很容易区分开来。Apodemus draco and A.peninsulae are the two species quite similar in morphological characters. In order to explore some diagnostic means of the biochemical taxonomy of the two species, LDH, EST and SOD isozymes were isolated from leg muscles,breast muscles,hearts,kidneys,livers,spleens,lungs and brains of A. draco and A.peninsulae and were analyzed by isoelectric focusing with PAGE. Distinct interspecific differences were found in EST and SOD electrophoregrams,but not found in LDH electrophoregrams.It was suggested that A.draco and A.peninsulae could be unequivocally distinguished by the presence or absence of A 2 band in EST isozymes or by the variations of isoelectric point(pI)of main bands in SOD isozymes.
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