TDZ对巨尾桉(GL9)胚性愈伤组织诱导和再生的影响  

Embroyogenic Callus Induction and Plant Regeneration of Clones GL9 of Eucalyptus grandis×E.urophylla

作　　者：裘珍飞[1] 曾炳山[1] 李湘阳[1] 刘英[1] 

机构地区：[1]中国林业科学研究院热带林业研究所,广东广州510520

出　　处：《林业科学研究》2009年第5期740-743,共4页Forest Research

基　　金：国家林业局948项目"桉树转基因技术及抗病育种基因质粒引进"(2006-4-70);科研院所基本科研费项目"桉树转基因技术及转基因育种研究"(2007-23)

摘　　要：The embryogenic callus induction and shoot regeneration were studied systematically in elite clone GL9 of Eucalyptus cultivated widely in south China.We investigated TDZ 0.02-0.05 mg·L-1 was the proper concentration.The callus induction rate was 92.9%—100%.When TDZ concentration was higher than 0.1 mg·L-1,the axillary bud germination was completely inhibited.TDZ 0.02 mg·L-1 with combinations of CoCl2 0.125 mg·L-1 could induct the embryogenic callus.The direct regeneration rate was 13.39%±1.03%,and with combinations of NAA 0.1mg·L-1 could not differentiate directly from callus,but higher regeneration rate(20.2%±13.3%) could be obtained by transferring callus onto regeneration medium.The size of callus can increase to 1.4 fold of its original size in the first subculture in modified MS+TDZ 0.02 mg·L-1 +NAA 0.1 mg·L-1medium and the average number of deep-pink-coloured masses of embryogenic cells on each callus was 8.4.In the second and third subculture,callus stopped growing further and the number of masses of embryogenic cells decreased gradually.Regeneration system could lay a good foundation for further transformation research.The embryogenic callus induction and shoot regeneration were studied systematically in elite clone GL9 of Eucalyptus cultivated widely in south China. We investigated TDZ 0. 02 - 0. 05 mg · L^-1 was the proper concentration. The callus induction rate was 92.9%--100%. When TDZ concentration was higher than 0.1 mg · L^-1, the axillary bud germination was completely inhibited. TDZ 0.02 mg· L^-1 with combinations of COC120. 125 mg· L^-1 could induct the embryogenic callus. The direct regeneration rate was 13.39% - 1.03%, and with combinations of NAA 0.1 mg· L^-1 could not differentiate directly from callus, but higher regeneration rate （20.2% - 13.3% ） could be obtained by transferring callus onto regeneration medium. The size of callus can increase to 1.4 fold of its original size in the first subculture in modified MS ＋ TDZ 0.02 mg · L^-1 ＋ NAA 0.1 mg · L^-1 medium and the average number of deep-pink-coloured masses of embryogenic cells on each callus was 8.4. In the second and third subculture, callus stopped growing further and the number of masses of embryogenic cells decreased gradually. Regeneration system could lay a good foundation for further transformation research.

关 键 词：TDZ 巨尾桉GL9 愈伤诱导率 再生率 

分 类 号：S792.39[农业科学—林木遗传育种]