小杂鱼携带细菌与大菱鲆疾病发生的相关性  

Correlation between bacteria associated with trash fish and diseases of cultured turbot (Scophthalmus maximus)

作　　者：刘朝阳[1] 王印庚[1] 孙晓庆[2] 

机构地区：[1]农业部可持续利用重点开发实验室,中国水产科学研究院黄海水产研究所,山东青岛266071 [2]中国海洋大学海洋生命学院,山东青岛266003

出　　处：《南方水产》2009年第5期44-51,共8页South China Fisheries Science

基　　金：国家高技术研究发展计划(863计划)项目(2003AA622070);农业结构调整重大技术研究专项(04-11-01B)

摘　　要：腹水病是工厂化养殖大菱鲆的一种常见病和多发病,常造成鱼类大规模的死亡。2006年6月山东某大菱鲆Scophthalmus maximus养殖场发生大规模腹水病,通过对病鱼细菌的分离、纯化和培养,对养殖井水、养殖池水和所用饵料小杂鱼进行细菌追踪调查,将从该养殖场所用小杂鱼中分离得到的细菌RF-1与鱼体分离的细菌FS-1进行比较,并对其进行人工感染试验,结果证明这2株菌对健康大菱鲆都具有很强的致病性,而且感染症状完全一致。进而对这2株菌进行了API-ID32E鉴定、传统的生理生化鉴定和16S rRNA序列分析,发现小杂鱼携带细菌RF-1与病原菌FS-1具有一致性,均为溶藻弧菌(Virio alginolyticus)。最后对这2株细菌进行了药敏试验,为控制疾病的发生和蔓延提供用药指导。Ascites syndromes disease is a common and ubiquitous disease in the industrial culture with turbot, which often results in turbot＇s massive mortalities. In June 2006, an outbreak of ascites syndromes disease of the cultured turbot Scophthalmus maximus occurred in an indoor farm in Shandong Province of China. Through the isolation, purification as well as cultivation of the bacteria in the diseased fish, and by tracing the bacteria in the well water, rearing pond water along with the feeding trash fish, it was found that the bacterium RF-I which existed in the trash fish was similar to the bacterium FS-1 in the diseased turbot; artificial infection test demonstrated that the bacteria RF-I and FS-1 had the same infection symptoms for healthy turbots. Further researches such as traditional physiological and biochemical test, API-ID32E test as well as 16S rRNA sequence analysis were also carried out. The results showed that the bacteria RF-1 and FS-1 were both Virio alginolyticus. Finally, a drug sensitivity test on these two bacteria was conducted to provide medical instruction for prevention and cure of ascites syndromes disease.

关 键 词：小杂鱼 大菱鲆 腹水病 相关性 

分 类 号：S941.3[农业科学—水产养殖]