高效液相色谱法测定血府逐瘀泡腾片中芍药苷含量  

Determination of Paeoniflorin in Xuefuzhuyu Effervescent Tablet by HPLC

作　　者：孙静[1] 仲崇林[2] 刘诗月[2] 

机构地区：[1]吉林大学第二附属医院药剂科,吉林长春130041 [2]吉林省中医药科学院,吉林长春130021

出　　处：《中国药业》2009年第19期33-34,共2页China Pharmaceuticals

摘　　要：目的建立测定血府逐瘀泡腾片中芍药苷含量的高效液相色谱(HPLC)法。方法以Diamonsil C18柱(250mm×4.6mm,5μm)为色谱柱,甲醇-水(35∶65)为流动相,检测波长为230nm。结果芍药苷进样量线性范围为0.386～1.930μg(r=0.9999),平均回收率为98.20%,RSD=0.74%(n=6)。结论该法操作简便,测定结果准确,精密度和稳定性良好,能有效地控制血府逐瘀泡腾片的质量。Objective To develop a HPLC method for determination performed on Dimonsil C18 column with CH3OH-H2O（35：65）as the range of paeoniflorin was 0. 386 μg - 1. 930 μg/mL（ r = 0. 999 9）. The Conclusion The method is simple and accurate, which can be used of paeoniflorin in Xuefuzhuyu Effervescent Tablet. Methods HPLC was mobile phase. The detection wavelength was 230 nm. Results The linear average recovery rate of paeoniflorin was 98.20%, RSD =0. 74% （n=6）. for the quality control of Xuefuzhuyu Effervescent Tablet.

关 键 词：高效液相色谱法 血府逐瘀泡腾片 芍药苷 

分 类 号：R284.1[医药卫生—中药学] R286.0[医药卫生—中医学]