鳗鲡冠状病毒样病毒的细胞分离与培养  

CELL ISOLATION AND CULTURE OF ANGUILLA ANGUILLA CORONAVIRUS LIKE VIRUS

作　　者：岳玉环[1] 黎诚耀[1] 杨盛华[1] 卢强[1] 陶增思[1] 王文东 邹啸环[1] 杨振国[1] 王振英[1] 殷震[1] 

机构地区：[1]解放军农牧大学水产动物疾病研究中心

出　　处：《水产学报》1998年第3期230-233,共4页Journal of Fisheries of China

摘　　要：实验选用４种鱼类传代细胞，对首次发现的鳗鲡“狂游病”的病原鳗鲡冠状病毒样病毒进行了细胞分离与培养。本研究在鲤上皮乳头状瘤细胞（ＥＰＣ）中复制了鳗鲡冠状病毒样病毒粒子，并经病毒细胞培养物的电镜负染和超薄切片法检查得到了证实。In this experiment,4 of the fish cell lines were selected to isolate and culture Eel Coronavirus like Virus that was a pathogen of eel(Anguilla anguilla)“mad swim disease”.The results showed that the Eel Coronavirus like Virus could be replicated in epithelioma papillosum cyprini (EPC).The growth of the virus in EPC was confirmed by electron microscopy method both in negative staining and ultrathin section specimens from observation of theviral cell cultures. And it was also verified tentatively that rainbow trout liver cells(R 1),grass carp kidney cells (GCK) and grass carp oavry cells(CO) were not sensitive to this virus.

关 键 词：欧洲鳗鲡 冠状病毒 样病毒 细胞培养 

分 类 号：S943.223[农业科学—水产养殖]