人抑制素合成肽与猪精液抑制素提取物主动免疫对母羊生殖激素妊娠及产羔的影响  

Effects of active immunization against a synthetic peptide sequence of the α subunit of human inhibin or partially purified inhibin from porcine seminal plasma on gonadotrophin and lambing rate in ewes

作　　者：张小冬[1,2] 程瑞禾[1,2] 夏银[1,2] 

机构地区：[1]南京农业大学动物科技学院 [2]南京师范大学生物系

出　　处：《南京农业大学学报》1998年第4期66-71,共6页Journal of Nanjing Agricultural University

基　　金：国家自然科学基金;江苏省自然科学基金

摘　　要：研究了抑制素主动免疫对母羊促卵泡素（ＦＳＨ）、促黄体素（ＬＨ）、孕酮（Ｐ）、雌二醇（Ｅ２）以及妊娠、产羔的影响。１５只发情季节考力代母羊平均分成３组：人抑制素α亚基（１－２６）Ｔｙｒ·Ｇｌｙ合成肽免疫组（Ｈ组）、猪精液抑制素免疫组（Ｓ组）和对照组（Ｃ组）。Ｈ组在初次免疫后即产生了抑制素抗体，第２次加强免疫后抗体滴度最高可达１∶６４０００。Ｓ组和Ｃ组均未产生可与人抑制素合成肽结合的抗体。Ｈ组母羊在第一次加强免疫后，血清ＦＳＨ和Ｐ的水平显著高于Ｃ组（Ｐ＜０．０５）。Ｓ组母羊Ｅ２浓度在初次和第一次加强免疫后显著高于对照组（Ｐ＜０．０５）。各组血清ＬＨ水平未有显著变化。Ｈ组和Ｓ组母羊妊娠期分别为１４８．６±１．６７ｄ和１５０．４±１．５２ｄ，受胎率１００％，与Ｃ组无显著差异。Ｈ组产羔率比Ｃ组增加４０％。结果提示，抑制素主动免疫在一定程度上免疫中和了抑制素的某些旁分泌或自分泌作用。Fifteen Corriedale ewes were actively immunized against a synthetic peptide sequence of human inhibin α subunit (group H) or partially purified inhibin from porcine seminal plasma (group S) in oestrous period. Control group (group C) were not immunized. All in gorup H produced antibodies that bound 1 26 peptide fragment of human inhibin α subunit while other two groups were not. After the first booster immunization the serum levels of FSH and progesterone in group H were significantly higher than those of group C ( P <0.05). The estradiol content of group S were significantly higher compared with control group. Serum LH concentration in all ewes did not change at each time of blooding. Inhibin immunization had no adverse effect on estrous, conception rate(100%) or length of gestation. A 40% increase in lambing rate was recorded for ewes in group H, indicating that inhibin immunization play a role in promoting the ovulation rate of these ewes. However, the mechanism of the increased ovulating rate in inhibin immunized ewes is still unclear. Immunization against inhibin may neutralized some autocrine or paracrine effects of inhibin, disinct from the systemic effect on FSH secretion.

关 键 词：抑制素 主动免疫 母羊 促性腺激素 产羔率 

分 类 号：S826.3[农业科学—畜牧学]