4种检测超广谱β-内酰胺酶方法的比较  

Comparison of Four Different Methods for Detection of Extended-spectrum Beta-lactamases

作　　者：杜军[1] 李敏[1] 崔景荣[2] 朱永红[2] 王玉炯[1] 

机构地区：[1]宁夏大学生命科学学院西部特色生物资源保护与利用教育部重点实验室,宁夏银川750021 [2]北京大学医学部,北京100083

出　　处：《宁夏大学学报（自然科学版）》2009年第3期268-270,281,共4页Journal of Ningxia University(Natural Science Edition)

基　　金：国家重点基础研究发展计划"973"项目(2006CB504400);高等学校科技创新工程重大项目培育基金资助项目(706057);北京大学天然药物及仿生药物国家重点实验室开放课题资助项目;宁夏大学自然科学研究基金资助项目(ZR200730)

摘　　要：以头孢噻肟、头孢他啶、头孢曲松和氨曲南为指示底物,用双纸片协同实验、纸片表型确证实验、三维实验和E-test法4种方法检测45株产超广谱β-内酰胺酶(ESBLs)阳性和59株产ESBLs阴性的大肠埃希菌和肺炎克雷伯菌.结果发现,4种方法的敏感性依次为95.56%,97.78%,93.33%,97.78%,特异性依次为96.61%,100.00%,98.31%,100.00%;头孢噻肟为宁夏地区用于检测ESBLs的最适指示底物;纸片表型确证实验和E-test法是4种检测方法中用于检测产ESBLs较可靠的方法,用三维实验检测产ESBLs菌株适合标本量较少的小型医院,双纸片协同实验和纸片表型确证实验在所有医院都适用.To Detect 45 strains of producing extended-spectrum beta-lactamase （ESBLs） and 59 strains nonproducing ESBLs in Escherichia coli and Klebsiella pneumoniae with double-disc synergy test, disc diffusion confirmatory test, three-dimensional test and E test, ceftazidime,cefotaxime,ceftriaxone and aztreonem were used as indicators. The sensitivity was 95.56% ,97.78% ,93.33% and 97.78% ,the specificity was 96.61% ,100.00%, 98.31%and 100. 00%, respectively. Cefotaxime was considered to be the better indicator. The disc diffusion confirmatory test and E-test were more reliable methods. The double-disc synergy test and disc diffusion confirmatory test were more convenient and suitable for the routine screening in hospital.

关 键 词：超广谱Β-内酰胺酶 大肠埃希菌 肺炎克雷伯菌 

分 类 号：R37[医药卫生—病原生物学]