隐匿性乙型肝炎病毒感染者血清HBsAg阴性原因探讨  

Studying on the Cause of HBsAg Being Negative in Sera from Patients with Occult Hepatitis B Virus Infection

作　　者：杨朝国[1] 张玲英[2] 许建[3] 

机构地区：[1]成都中医药大学医学技术学院,四川成都610041 [2]四川省人民医院,四川成都610072 [3]成都市第九人民医院,四川成都610041

出　　处：《四川省卫生管理干部学院学报》2009年第2期103-104,115,共3页Journal of Sichuan Continuing Education College of Medical Sciences

基　　金：四川省卫生厅科研课题(编号060093)

摘　　要：目的:探讨隐匿性乙型肝炎病毒(HBV)感染者血清HBsAg阴性的原因。方法:用高灵敏的电化学发光免疫技术对61例隐匿性HBV感染者血清HBsAg进行定量测定,同时用荧光定量PCR对血清HBV-DNA进行定量检测。结果:61例隐匿性乙型肝炎病毒感染中,血清HBsAg未检出有8例,在0.05ng/ml^0.1ng/ml有27例,0.1ng/ml^0.5ng/ml有21例,0.5ng/ml^10ng/ml有5例。隐匿性HBV感染者血清HBV DNA水平大多数低于103copies/ml。结论:隐匿性HBV感染者血清HBsAg阴性的原因主要是HBV复制和基因表达的严重抑制。Objective： To investigate the cause of HBsAg being negative in sera from patients with occult hepatitis B virus infection. Methods： HBsAg in the sera from 61 cases of patients with occult hepatitis B virus infection was quantitatively detected by electro- chemiluminescence immunoassay （ ECLIA）. And HBV-DNA in the sera was determined by QF-PCR. Results： In the 61 cases with occult hepatitis B virus infection, 8 cases were negative, Quantities of HBsAg in the sera from 27 cases was from 0.05ng/ml to 0.1ng/ml, 21 cases, 0.1ng/ml to 0.5ng/ml, 5 cases, 0.5ng/ml to 10ng/ml. Quantities of HBV DNA in the sera were lower than 103 copies/ml. Conclusion： HBsAg being negative in sera from patients with occult hepatitis B virus infection was mostly due to a severe suppression of HBV replication and gene expression.

关 键 词：隐匿性乙型肝炎病毒感染 巢式聚合酶链反应 乙型肝炎病毒核酸 乙型肝炎表面抗原 

分 类 号：R512.62[医药卫生—内科学]