攀西地区葛藤根瘤菌的RFLP遗传多样性  

RFLP Genetic Diversity of Rhizobia Isolated from Pueraria lobata in Panxi

作　　者：谢徽[1] 张小平[1] K.Lindstrom 

机构地区：[1]四川农业大学资源环境学院,四川雅安62500 [2]赫尔辛基大学应用化学与微生物学系,芬兰赫尔辛基00014

出　　处：《四川农业大学学报》2009年第3期314-320,共7页Journal of Sichuan Agricultural University

基　　金：国家自然科学基金(No.30570062)

摘　　要：利用16S rDNA PCR-RFLP和16-23S IGS PCR-RFLP技术对分离自四川攀枝花的43株葛藤根瘤菌进行了遗传多样性研究。供试菌株的16S rDNA用HaeⅢ、MspⅠ、HinfⅠ和TaqⅠ酶切后具有16种遗传图谱类型。16S-23S rDNA IGS PCR-RFLP分析结果表明,在53%的相似性水平上,供试菌株与参比菌株分为两个分支,在81%的相似水平上所有菌株分为13个群。Forty-three Rhizobia strains were isolated and analyzed by using 16S rDNA and 16-23S rDNA intergernic spacer （IGS） PCR-RFLP, and the PCR fragments were digested by restriction endonucleases including HaeⅢ, Msp Ⅰ , HinfⅠ and Taq Ⅰ . The results showed that 16S rDNA PCR-RFLP divided all the strains into 16 genotypes, and the results of 16-23S rDNA PCR-RFLP showed that all the strains clustered 2 geno-branehes at the similarity of 53%, and were divided into 13 groups at the similarity of 81%.

关 键 词：根瘤菌 葛藤 16S RDNA PCR RFLP 16—23S IGS PCR RFLP 遗传多样性 

分 类 号：S54[农业科学—作物学]