2,2’,4,4’-四溴联苯醚对小鼠甲状腺激素系统稳态的影响及作用机制研究  

Effects and toxicity mechanism of 2,2',4,4'-tetrabrominated diphenyl ethers on the homeostasis of thyroid hormone system in C57BL/6 mice

作　　者：刘早玲[1] 刘继文[1] 张建清[2] 田亚楠 方道奎[2] 黄海燕[2] 蒋友胜[2] 周健[2] 张红宇[2] 梅树江[2] 

机构地区：[1]新疆医科大学公共卫生学院,乌鲁木齐830054 [2]深圳市疾病预防控制中心 [3]美国德克萨斯州A&M大学

出　　处：《卫生研究》2009年第5期522-524,共3页Journal of Hygiene Research

基　　金：国家自然科学基金面上项目(No.30771778)

摘　　要：目的研究不同剂量2,2’,4,4’-四溴联苯醚(BDE-47)对C57BL/6小鼠甲状腺激素T3、T4和促甲状腺激素TSH的影响,并探讨可能的毒作用机制。方法将C57BL/6小鼠分成对照组和低、中、高剂量组,对照组按0.1ml/10g给予玉米油,染毒组分别按0.5、5和50mg/kg剂量给予BDE-47化合物,连续4天腹腔注射后,收集动物血液、肝脏和甲状腺组织。电化学发光免疫法测定各组小鼠血清总T4(TT4)、总T3(TT3)和TSH,愈创木酚法测定甲状腺组织中过氧化物酶(TPO)活性,CHOPRA法检测肝脏中I型脱碘酶(DI)活性。结果与对照组相比,所有染毒剂量组TT4水平均降低(P<0.01),中、高剂量染毒组小鼠TT3水平降低(P<0.01);中、高剂量染毒组小鼠肝脏DI活性与对照组相比有显著增加(P<0.05);血清TSH水平和甲状腺TPO活性各组之间无显著差异(P>0.05)。结论BDE-47可以破坏小鼠甲状腺激素系统稳态,产生甲状腺激素干扰毒性,其作用机制之一可能是影响肝脏DI活性。Objective To investigate the effects and of toxicity mechanism of 2,2＇ ,4,4＇-TETRABDE （BDE-47） on TT3, TT4 and TSH in C57BL/6 mice. Methods C57BL/6 mice were divided randomly into 4 groups, and given with corn oil（control group） and 1μmol/（kg· d）, 10μmol/（ kg· day） and 100μmol/（kg·day） BDE-47 for 4 days through intraperitoneal injection respectively. After collecting the bloods, livers and thyroids, the T3, T4 and TSH levels of serum were detected by electro chemiluminescenc（ECLI）, thyroid peroxidase（TPO） activities in thyroid by guaiacol method and type- Ⅰ deiodinase（DI） in liver by CHOPRA method. Results Compared with the control group, the level of TT4 decreased significantly in the three BDE-47 dose groups, TT3 in the medium and high dose groups decreased significantly, and the activities of DI increased in the medium and high dose groups significantly（ P 〈 0.05）, whereas TSH level in serum and activity of TPO in thyroid in the three dose groups weren＇ t statistically significant comparing with control group. Conclusion BDE-47 could affect the homeostasis of thyroid hormone system, and activity of type-Ⅰ deiodinase（DI） in liver being changed might be the one of toxicity mechanisms

关 键 词：2 2' 4 4'-四溴联苯醚 三碘甲腺原氨酸 甲状腺素 I型脱碘酶 甲状腺过氧化物酶 

分 类 号：Q575.1[生物学—生物化学] R322.51[医药卫生—人体解剖和组织胚胎学]