用去氧胆酸盐和β-环糊精的毛细管胶束电动色谱拆分手性药物  

Enantioseparation of Chlorpheniramine and EMD 56431 by Micellar Electrokinetic Capillary Chromatography Using Deoxycholate Salt and β Cyclodextrin

作　　者：许旭[1,2] 张梅[1,2] 吴如金[1,2] 林炳承[1,2] 

机构地区：[1]中国科学院大连化学物理研究所 [2]中国药科大学

出　　处：《色谱》1998年第5期379-382,共4页Chinese Journal of Chromatography

基　　金：国家自然科学基金

摘　　要：用β－环糊精（β－ＣＤ）和去氧胆酸钠（ＳＤＣ）的环糊精改性毛细管胶束电动色谱，实际拆分了ＥＭＤ－５６４３１和扑尔敏两种手性药物，研究了ＳＤＣ和β－ＣＤ浓度及ｐＨ值对分离的影响。初步讨论了分离的机理。认为ＣＤ－ＳＤＣ体系中胶束单体分子几乎均被ＣＤ包合；ＣＤ可能与部分胶束单体包合而存在于ＳＤＣ的胶束中；该拆分体系中ＳＤＣ与β－ＣＤ的浓度比在４∶１～４∶３时拆分效果最好。并发现ＳＤＣ对β－ＣＤ有显著的增溶作用。Two chiral drugs of EMD 56431 and chlorpheniramine are separated by cyclodextrin modified micellar electrokinetic capillary chromatography using β cyclodextrin( β CD ) and sodium deoxycholate(SDC) as selector in the paper. All the electrolytes in experiments contain 0.1mol/L borate. The length of capillary is 45.0cm as total and 30.0cm as effective. The running and loading voltages are all 7.8kV. The effects of pH and concentrations of SDC and β CD  are studied, in which the best chiral separation conditions for EMD 56431 are pH 10.4, [β CD ]=50mmol/L, =150mmol/L, and those for chlorpheniramine are pH 9.0, [β CD ]=50mmol/L, =100mmol/L. The mechanism of chiral separation for the buffer system is initially believed as: the micellar monomer exists almost all as inclusion body with β CD, some CD SDC inclusion complex may exist in micellar because the SDC micellar′s gather number is only 4 and the SDC molecular is so big that it can only partly enter β CD. Then,the good separation ability of the system is supplied while the ratio of concentration between SDC and β CD is in 4:1-4:3;but there will be a optimized total concentration for SDC and β CD.The complex interaction among sample, SDC and β CD makes intricate change for migration along with the selector′s concentration,and the same complex results are also made in pH experiments because of electroosmosis and the acidity of SDC and components. The phenomenon of increasing β CD solubility is showed. The β CD′s solubility with 100 mmol/L SDC can be increased above 150 mmol/L. 

关 键 词：环糊精 去氧胆酸钠 扑尔敏 拆分 EMD-56431 MEKC 

分 类 号：R976[医药卫生—药品]