手性毛细管色谱法测定人尿中美芬妥英对映体的含量及其在代谢分型中的应用  

Determination of Mephenytoin Enantiomers in Human Urine Using Chiral Capillary Chromatography and Its Application in Metabolism Polymorphism Study

作　　者：姚彤炜[1] 曾苏[1] 阮宏强[1] 陈枢青[1] 

机构地区：[1]浙江医科大学药物分析教研室1浙江医科大学生化教研室

出　　处：《色谱》1998年第5期408-410,共3页Chinese Journal of Chromatography

基　　金：浙江省自然科学基金

摘　　要：采用手性毛细管色谱柱和ＦＩＤ检测器建立了人尿中美芬妥英（ＭＰ）对映体的定量分析方法。尿样用二氯乙烷提取，用酸、碱洗涤得以纯化，测得各对映体的最低检测限为６０μｇ／Ｌ。在１１５～６９０μｇ／Ｌ浓度范围内，标准曲线呈良好的线性关系，ｒ＞０．９９，日内、日间精密度ＲＳＤ＜６．５％，Ｓ－ＭＰ的平均回收率为７４．４１％，Ｒ－ＭＰ的平均回收率为７３．７８％。并以ＭＰ为探针药物，对３２名志愿者的尿样进行了ＭＰ氧化代谢分型研究。The determination of mephenytoin enantiomers in human urine using chiral capillary chromatography is described. MP in urine was extracted with using dichloroeth ane and the organic layer was washed with acidic and basic aqueous solution. The organic phase was evaporated to dryness under nitrogen on water bath (55℃). The residue was dissolved in 10μL ethyl acetate and 1μL was injected into the GC. MP enantiomers was separated on a Chirasil Val SFOT column with nitrogen as carrier gas and FID as detector. The linear relationship was obtained over the concentration range of 115 690μg/L (r=0 991 3 for S MP and r=0 993 4 for R MP) with a detection limit of 60ng MP enantiomers/mL urine. The recoveries were 74 41% for S MP and 73 78% for R MP. The relative standard deviation within day and between days were less than 6 5%. The method was used to study the metabolism polymorphism of 32 volunteers. The S/R ratio was calculated to express interindividual variation in metabolism, in which, the S/R ratio of 5 subjects was more than 0 95, as the poor metabolizers and that of the others was less than 0 8, as the extensive metabolizers.

关 键 词：手性毛细管色谱 美芬妥英 代谢分型 尿液 分析 

分 类 号：R971.6[医药卫生—药品]