气相色谱法测定冠心膏中樟脑、薄荷脑、异龙脑、龙脑的含量  

Gas Chromatographic Determination of Camphora, Mentholum, Isoborneol and Borneol in Guanxingao

作　　者：阎正[1,2] 刘鹏岩[1,2] 张建申[1,2] 王长宁 冯天铸[1,2] 周文英[1,2] 

机构地区：[1]河北大学化学系 [2]河北省职工医学院

出　　处：《色谱》1998年第5期411-413,共3页Chinese Journal of Chromatography

摘　　要：用气相色谱法测定了冠心膏中樟脑、薄荷脑、异龙脑和龙脑的含量。固定相：７％ＰＥＧ－１５００，线性范围：５～４５０ｍｇ／Ｌ（ｒ＝０．９９９４～０．９９９９）。回收率：樟脑为９８．４４％，薄荷脑为９９．０１％，异龙脑为９９．６９％，龙脑为１０１．９％。Guanxingao is a kind of traditional Chinese rubber electuary medicine which is able to either cure or guard against coronary heart disease and angina pectoris. The contents of camphora, mentholum, isoborneol and borneol in Guanxingao are determined by gas chromatography. The purpose of the study is to detect and control the loss of the four volatile components through production and standing and to guarantee the curative effect. Chromatographic analysis was performed on a GC 4004 gas chromatograph(FID). The column was a 3mm i.d. ×2m stainless steel tube packed with 7% PEG 1500 on 100 110mesh 102 non silanized white support. The column and the FID temperatures were 115℃ and 180℃ respectively. H 2 was the carrier gas, 30 mL/min. Internal standard method was used for the quantitative estimation with naphthalene as the internal standard. The linear ranges were at least within 50 450mg/L (r=0.999 9, n=4). The correction factors against naphthalene were 1.262 1.286 and the RSDs were 0.32% 1.5%(n=12). The recoveries were 98.44% 101.9%. In comparison with the theoretical contents, the average loss percentages are 71.72% (camphora), 65.60% (mentholum) and 66.31% (isoborneol+borneol). The samples were pretreated by means of isothermal (35℃) water bath extraction with acetone for 6 times with four hours each.

关 键 词：气相色谱 樟脑 薄荷脑 异龙脑 龙脑 冠心膏 分析 

分 类 号：R972.4[医药卫生—药品]