高效液相色谱手性流动相添加剂分离肾上腺素类对映体  

Enantiomeric High Performance Liquid Chromatographic Separation of Epinephrine and Analogous Using Chiral Mobile Phase Additives

作　　者：李桦[1] 胡先明[1] 谢颖峰 

机构地区：[1]武汉大学分析测试科学系

出　　处：《色谱》1998年第5期424-426,共3页Chinese Journal of Chromatography

摘　　要：将β－环糊精、２，６－二甲基－β－环糊精、２，３，６－三甲基－β－环糊精分别作为手性流动相添加剂，较系统地研究了Ｄ，Ｌ－肾上腺素、Ｄ，Ｌ－异丙肾上腺素、Ｄ，Ｌ－麻黄碱在反相ＨＰＬＣ系统中的拆分，建立了甲基化β－环糊精动态手性固定相法分离肾上腺素类药物对映体的方法。The enantiomeric resolution of epinephrine, isoproterenol, ephedrine was studied by reversed phase HPLC. β CD, DM β CD,TM β CD were used as chiral mobile phase additives. The effects of the concentration of DM β CD and the concentration of methanol on resolution of D,L epinephrine were investigated. The results showed: D,L epinephrine and D,L isoproterenol can be separated by β CD, DM β CD, TM β CD, but D,L ephedrine cant. Hydrogen bonding plays an important role in the chiral recognition mechanism. In the separation of isoproterenol, the enantioselectivity of TM β CD was better than those of β CD and DM β CD. As the concentration of DM β CD changed from 0 04mmol/L to 1 00mmol/L, the resolution varied slightly. The experiment of the effect of methanol concentration on R s showed: when the ratio of methanol/water was 40/60, the R s reached the maximum. The coefficients of variation of retention time of D epinephrine and L epinephrine were 1 3% and 1 4% respectively. It is demonstrated that the chromatographic systems with a dynamically generated stationary phase with methylated β cyclodextrin are versatile tools for enantiomer separation. This technique leads to column with excellent time stability and good reproducibility of the enantio selectivity.

关 键 词：HPLC 对映体 拆分 肾上腺素 拟肾上腺素 

分 类 号：R977.11[医药卫生—药品]