橡胶树RRIM600品系的内生真菌多样性  

Diversity of Endophytic Fungi from RIMM600,a Clone of Hevea brasiliensis

作　　者：李晓娜[1] 李增平[1] 郑服丛[1] 

机构地区：[1]海南大学环境与植物保护学院,海南儋州571737

出　　处：《热带作物学报》2009年第7期990-994,共5页Chinese Journal of Tropical Crops

基　　金：海南大学科技基金项目(No.Rnd0719);国家支撑计划子课题项目(No.2007BA048B04-011)资助

摘　　要：从海南省儋州市的橡胶树RRIM600品系上,采集花、叶、枝、果、根组织分离内生真菌,共分离了504个组织块,获得内生真菌545株。根据形态特征对482株产生孢子的菌株进行鉴定,分别归入21个属,大部分为半知菌,共19属,其中丝孢纲真菌12属,腔孢纲真菌7属;少数为子囊菌,共2属。初步研究结果表明:拟茎点霉属(Phomopsis)为橡胶树5种组织中的优势属,刺盘孢属(Colletotrichum)为叶片组织中的优势属,侧多隔孢属(Pleurophragmium)为根组织中的优势属。这5种组织中的内生真菌定殖率为50%～100%、分离率为0.74～1.52。内生真菌群落组成的相似性比较结果表明,部分内生真菌具有一定的组织偏好性。Tissues of leaves, branches, roots, flowers and fruit of rubber tree RIMM600, a clone of Hevea brasiliensis, were collected in Danzhou, Hainan Province, from which endophtyic fungi were isolated. A total of 545 strains of the endophytic fungi were obtained from 504 tissue segments isolated. According to their morphological characteristics, 482 strains were identified and classified into 21 genera, 19 of which were Imperfect fungi： 12 belonged to Hyphomycetes and 7 to Coelomycetes, 2 of which were Ascomycetes. Phomopsis was a dominant genus in the 5 tissue organs of the rubber tree, Colletotrichum a dominant genus in the leaf tissue, and Pleurophrogmium a dominant genus in the root. The colonization rate in the 5 tissue organs among the samples ranged from 50% to 100% rate from 0.74 to 1.52. Similarity comparison among the endophytic fungal communities endophytic fungi had a preference to their hosting tissues.

关 键 词：内生真菌 橡胶树 RRIM600品系 多样性 

分 类 号：S794.1[农业科学—林木遗传育种]