氟致人肝细胞DNA损伤与细胞凋亡关系的研究  

Study on Relationship Between DNA Damage and Apoptosis Induced by Fluoride in Human Hepatocytes

作　　者：褚启龙[1] 哈建利[1] 王爱国[2] 

机构地区：[1]湖北职业技术学院医学院,湖北孝感432000 [2]华中科技大学同济医学院公共卫生学院环境卫生学教研室

出　　处：《实用预防医学》2009年第5期1347-1348,共2页Practical Preventive Medicine

基　　金：国家自然科学基金资助项目(编号:30271155)

摘　　要：目的研究氟致人肝细胞DNA损伤与细胞凋亡之间的关系。方法采用40、80、160μg/ml氟化钠对培养的人肝细胞进行染毒,12h后用单细胞凝胶电泳技术(SCGE)和流式细胞术(FCM)检测氟化物对人肝细胞DNA损伤和细胞凋亡情况。结果各氟染毒组人肝细胞DNA损伤程度明显高于对照组,并且随着染氟剂量的增高而增大;中、高剂量氟组人肝细胞凋亡百分率均明显高于对照组。同时,高剂量氟组与低剂量氟组之间细胞DNA损伤程度和凋亡率的差异亦有统计学意义。结论氟可导致人肝细胞DNA损伤,诱导细胞凋亡,一定浓度范围内的氟所导致的DNA损伤与细胞凋亡之间呈现明显的正相关关系。Objective To explore the relationship between DNA damage and apoptosis in human hepatocytes induced by fluoride. Methods DNA damage and apoptosis were measured after human hepatocytes were incubated with 40,80, and 160 μg/ml sodium fluoride using Single Cell Electrophosis （SCGE） and Flow Cytometry（FCM）, respectively. Results The DNA damage of human hepatocytes in the fluoride groups were significantly higher than that of the control group. The percentages of apoptosis in the middle and high fluoride groups were significantly higher than that of the control group. Moreover, the level of DNA damage and the percentage of apoptosis in high- fluoride group were significantly higher than those of the low fluoride group. Conclusions Fluoride exposure can cause DNA damage and apoptosis in human hepatocytes. Furthermore, there exists a significant positive correlation between DNA damage and apoptosis induced by fluoride in certain level range.

关 键 词：氟 人肝细胞 DNA损伤 细胞凋亡 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]