乙肝患者前S1抗原与HBeAg及HBV-DNA检测的关系  

Correlation Among PreS1,HBeAg and HBV-DNA in Patient with Hepatitis B

作　　者：叶文仙[1] 吴意[2] 

机构地区：[1]贵州省贵阳市第四人民医院,贵州贵阳550002 [2]湖南省人民医院

出　　处：《实用预防医学》2009年第5期1397-1399,共3页Practical Preventive Medicine

摘　　要：目的探讨HBV前S1抗原与HBeAg、HBV-DNA三者的相关性关系,分析PreS1Ag检测在判断乙型肝炎病毒复制中的作用。方法应用ELISA法对340份HBsAg阳性血清和100份健康体检(HBsAg阴性血清)进行PreS1-Ag和HBV血清标志物检测,并用实时荧光定量PCR检测HBV-DNA结果进行比较。结果在检测的340份HB-sAg阳性血清中,PreS1Ag阳性率为60.2%(205/340),HBeAg阳性率为43.5%(148/340),HBV-DNA阳性率为59.7%(203/340)。在148份HBeAg阳性血清中,PreS1Ag阳性率79.7%(118/148),HBV-DNA阳性率84.4%(125/148);192份HBeAg阴性血清中,PreS1Ag阳性率45.3%(87/192),HBV-DNA阳性率为40.6%(78/192)。HBeAg与HBV-DNA、PreS1的阳性率差异有统计学意义(P<0.05),HBeAg阳性血清中PreS1Ag(79.7%)阳性率显著高于HBeAg阴性血清PreS1Ag(45.3%)阳性率(P<0.01);PreS1抗原与HBV-DNA检出率有密切的相关性。340份HBsAg阳性血清中HBeAg、PreS1和HBV-DNA阳性数(率)分别为148(43.5%)、205(60.2%)、203(59.7%);100份HBsAg阴性血清中PreS1和HBV-DNA阳性数(率)分别为3(3%)、2(2%)。结论前S1抗原在乙肝诊断中是一个很重要的指标,它能够反映HBV复制的情况,PreS1Ag较HBeAg更敏感,两者比较差异有统计学意义(P<0.05)。前S1抗原与HBV-DNA阳性呈高度正相关。Objective To explore the correlation among HBV pre Sl antigen, HBeAg and HBV- DNA, and to analyze the role of PreS1Ag detection in judging hepatitis B virus replication. Methods ELISA method was used to detect PreS1Ag and HBV serological markers of 340 HBsAg positive sera and 100 HBsAg negative sera, HBV- DNA was detected by real - time fluorescence quantitative POR, and the detection results were compared. Results In the 340 HBsAg positive sera, PreS1Ag positive rate was 60.2 % （205/340）, HBeAg positive rate was 43.5 % （148/340） and HBV DNA positive rate was 59.7% （203/340）. Besides, in the 148 HBeAg positive sera, PreS1Ag positive rate was 79.7% （118/148） and HBV-DNA positive rate was 84.4% （125/148）, while in 192 HBeAg negative sera, PreS1Ag positive rate was 45.3% （87/192） and HBV - DNA positive rate was 40.6% （78/192）. The positive rates of HBeAg, HBV DNA and PreS1 were statistically significant different （P〈 0.05）, the positive rate of PreS1Ag （79.7%） in all of HBeAg positive sera was significantly higher than that （45.3%） in all of HBeAg negative sera （P〈 0.01）. PreS1 antigen was closely related to the detection rate of HBV- DNA. In the 340 HBsAg positive sera, the number （rate） of HBeAg, PreS1 and HBV- DNA was 148 （43.5 % ）, 205 （60.2 % ） and 203（59.7% ） respectively, while in the 100 HBsAg negative sera, the positive number （rate） of PreS1 and HBV- DNA was 3（3 % ） and 2 （2 % ） respectively. Conclusions Pre - S1 antigen is a significant maker in diagnosis of hepatitis B, which has the ability to respond to the case of HBV replication, Pre - S1 antigen is more sensitive than HBeAg, there is a statistically significant difference between the two detection methods（P〈 0.05）. The positive Pre- Sl antigen is positively cotrelated with the positive HBV- DNA.

关 键 词：乙肝病毒 前S1抗原 HBV-DNA HBEAG 

分 类 号：R373.21[医药卫生—病原生物学]