反义Waxy基因转化籼稻9311的初步研究  

Genetic transformation of indica rice 9311 with antisense Waxy gene

作　　者：丁盛[1,2] 刘清[1] 黄志刚[1] 周建军[2] 萧浪涛[1] 

机构地区：[1]湖南农业大学植物激素与生长发育湖南省重点实验室,湖南长沙410128 [2]湖南农业大学理学院,湖南长沙410128

出　　处：《湖南农业大学学报（自然科学版）》2009年第4期366-368,382,共4页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：湖南省应用基础研究计划重点项目(2007FJ2003);湖南农业大学人才科学基金项目(08YJ06);湖南农业大学青年科学基金项目(08QN20)

摘　　要：以籼稻品种9311为材料,研究了不同诱导培养基对籼稻9311的诱导率,以及农杆菌浓度、浸染时间和干燥处理时间对抗性愈伤率的影响,进而采用农杆菌介导的共转化方法将含有反义Waxy基因的p13W8质粒和含有潮霉素抗性标记基因的p1300质粒同时转化受体材料.结果表明:籼稻9311成熟胚在含质量浓度为3.0mg/L的2,4-D的N6基本培养基上具有较高的愈伤组织诱导率,且愈伤的胚性性状良好;愈伤组织在OD600值为0.6的农杆菌菌液中浸染10min、干燥1.5～2.0h处理后能够获得较多的抗性愈伤组织;对转化水稻的再生植株进行PCR检测表明,反义Waxy基因已整合进T0代水稻基因组中.The indica rice variety 9311 was used to study the inductions of different culture mediums on it, and the impact of Agrobacterium concentration, infected time and drying time on confrontational callus. Then by applying co-transformation method, plasmid pl3W8 which contained antisense Waxy gene and plasmid pl300 which contained marker gene were transformated into calli induced from matural embryos by Agrobacterium tumefaciens. The results show that N6 medium is the best suitable medium for callus inducing from 9311. The suitable concentration of Agrobacterium is OD600=0.6 and the incursion time is 10 min, with 1.5-2.0 h desiccation after the incursion. And PCR analysis show that the antisense Waxy gene has been integrated into the genome of the regeneration plants.

关 键 词：籼稻 成熟胚 反义蜡质基因 

分 类 号：Q785[生物学—分子生物学]