增效灭鼠剂对大鼠作用机制研究及中毒鼠病理学观察  

Mechanism of synergistic rodenticide in rats and its pathology observation

作　　者：吴丁兰[1] 张国萍[1] 常弘[1] 冯志勇[2] 肖白玉[1] 姚丹丹[2] 杨登亮[1] 

机构地区：[1]中山大学生命科学学院,广东广州510275 [2]广东省农业科学院植物保护研究所

出　　处：《中国媒介生物学及控制杂志》2009年第5期413-415,共3页Chinese Journal of Vector Biology and Control

基　　金：广东省教育部产学研综合项目(2007B090400103)

摘　　要：目的研究增效灭鼠剂的增效作用机制,并观察实验性增效灭鼠剂对鼠类中毒后病理组织变化,为提高灭鼠效果和应用提供依据。方法以SD大鼠为模型,检测增效灭鼠剂对大鼠凝血时间(CT)、凝血酶原时间(PT)、部分凝血酶原时间(APTT)和纤维蛋白原(FIB)的影响,采用石蜡切片和HE染色技术观察大鼠实验性增效灭鼠剂中毒后的组织病理变化。结果单独增效剂能引起大鼠CT、APTT延长以及FIB的增加;杀鼠迷组与增效杀鼠迷组CT、PT、APTT均显著延长,FIB显著减少;相对于杀鼠迷组,增效杀鼠迷组CT、PT、APTT显著延长,FIB减少。中毒鼠内脏病理学观察发现,增效灭鼠剂能引起更大程度的胃肠表面黏膜破坏而导致溃疡,肺静脉血管出现破裂及肝组织损伤坏死。结论增效剂本身具有抗凝血功能,同浓度的增效杀鼠迷组比杀鼠迷组具有更强的抗凝血功能;增效灭鼠剂导致鼠类内出血并引起肝脏损伤,进一步阻碍肝脏凝血功能和其他正常生理功能,而增效剂本身并不会造成组织病理损伤。Objective To study the synergistic mechanism of synergistic rodenticides, and to observe the pathological changes in rodents tissue so as to provide the evidence for its application. Methods The effect of synergistic rodenticides to CT, PT, APTT and FIB were evaluated at the condition of SD rat as a model. The pathological changes in the tissues were detected by paraffin slice and HE staining technique. Results Single synergist could prolong CT and APTT and also increased FIB. While both warfarin and synergistic warfairn could obviously prolong CT, PT and APTT and reduced FIB. Compared to warfarin, the synergistic warfairn could increase CT, PT and APTT and reduced FIB. The histology and pathology observation indicated that synergistic warfairn could lead to a peptic ulcer compared with warfarin. It also led a pneumorrhagia and necrotic liver in toxic rats. Conclusion It suggests that synergist has an anticoagulant function, and the anticoagulant role of synergistic warfairn is powerful than that of traditional warfarin. Synergistic warfairn also leads to internal hemorrhaget and other physiological disfunction, while single synergist does not cause the histopathological damage.

关 键 词：增效灭鼠剂 抗凝血 病理组织观察 SD大鼠 

分 类 号：S443[农业科学—农业昆虫与害虫防治]