双抗原夹心ELISA检测鼠疫F1抗体技术的应用  

Application of double antigens sandwich enzyme linked immunosorbent assay(DAgS-ELISA) on the detection of Yersinia pestis F1 antibody

作　　者：刘合智[1] 张懿晖[1] 杨晓燕[1] 王海峰[1] 杜国义[1] 胡乐乐[1] 杨顺林[1] 董国润[1] 

机构地区：[1]河北省鼠疫防治所检验科,张家口075000

出　　处：《中国媒介生物学及控制杂志》2009年第5期467-469,共3页Chinese Journal of Vector Biology and Control

基　　金：河北省医学适用技术跟踪项目(GL200635)

摘　　要：目的研究双抗原夹心酶联免疫吸附试验(DAgS-ELISA)检测鼠疫F1抗体技术在鼠疫监测中的实用性。方法用DAgS-ELISA和间接血球凝集试验(IHA)微量法对比检测558份标本鼠疫F1抗体。结果IHA检测出阳性33份,DAgS-ELISA检出阳性31份,阳性符合率为90.91%,阴性符合率99.81%,总符合率99.28%,二者检出阳性率分别为5.91%和5.56%,差异无统计学意义(χ2=0.25,P=0.625)。2种方法测定27份鼠疫免疫血清均阳性,IHA微量法的敏感性高于DAgS-ELISA(t=3.023,P=0.006)。结论DAgS-ELISA检测鼠疫F1抗体敏感性低于IHA微量法,但特异性好,无前滞反应,可避免初筛漏检问题。Objective To study the practicability of double antigens sandwich enzyme linked immunosorbent assay （DAgS-ELISA）on the detection of Yersinia pestis F1 antibodies. Methods A total of 558 samples antibodies of anti-F1 antigen were detected by DAgS-ELISA and trace indirect hemagglutination assay （trace-IHA）. Results Thirty three samples were positive tested by IHA, 31 positive by DAgS-ELISA, the positive accordance rate was 90.91%, 99.81% for negative accordance rate, 99.28% for the total accordance rate. The positive rate detected by IHA and DAgS-ELISA were 5.91% and 5.56% respectively, and no statistics difference was found （X2=0.25, P=0.625）. About 27 the immuno-serum were positive detected by IHA and DAgS-ELISA methods, and the sensitivity of IHA test were all higher than that of DAgS-ELISA （t=3.023, P=0.006）. Conclusion Sensitivity of DAgS-ELISA is lower than that of trace-IliA, but its specificity is better and no primary inhibitory phenomena, and could exempt from leak detection in the preliminary screening.

关 键 词：酶联免疫吸附试验 鼠疫耶尔森菌 F1抗原 抗体 

分 类 号：R254.8[医药卫生—中医内科学]