高效液相色谱法测定银杏叶中2种活性成分含量  

Determination of Two Active Constituents in Folium Ginkgo by HPLC

作　　者：杨驰[1] 刘思曼[1] 

机构地区：[1]绵阳师范学院化学与化学工程学院,四川绵阳621000

出　　处：《绵阳师范学院学报》2009年第8期43-45,共3页Journal of Mianyang Teachers＇ College

基　　金：绵阳市科技局自然科学研究项目(2006S016)

摘　　要：建立高效液相色谱仪测定银杏叶中槲皮素、异鼠李素含量的方法。该方法采用:D iamonsilTMC18(4.6mm×250 mm,5μm)反相色谱柱,流动相甲醇-1%乙酸(45?55);流速0.9 m l.m in-1;检测波长365 nm。结果:2种黄酮苷元分离度良好(R>1.5),并通过重复性实验和加标回收实验,验证了方法的稳定性和可靠性。标准曲线相关系数r=0.9998;槲皮素、异鼠李素的平均回收率为98.9%、98.7%,RSD分别为1.6%、0.7%。Objective： To establish a HPLC method for the simultaneous quantification of quercetin and sorhamnetin in Folium Ginkgo. Methods： The samples are separated on a Diamonsil^TMC18 （4.6 mm×250 mm, 5μm） column with gradient elution and the mobile phase consisted of methanol and 1% acetic acid. The flow rate is 0. 9 mL · min - 1 and the detection wavelength is set at 365 nm. Results： Two active constituents are separated with good resolution （ R 〉 1.5）. The calibration curves exhibit excellent linear behavior （ r = 0.9998）. The reproducibility of the method is determined by repeated injection experiments. The recovery of the method is determined with the standard addition method, with results of 98.9 % for quercetin and 98.7 % for sorhamnetin. The relative standard deviations （ RSD ） are 1. 6% and 0.7%.

关 键 词：高效液相色谱 银杏叶 槲皮素 异鼠李素 

分 类 号：O657.7[理学—分析化学]