粘质沙雷氏菌XJ-01几丁质酶合成条件的优化  

Optimal Conditions for Chitinase Production by Serratia Marcescens XJ-01

作　　者：夏金兰[1] 熊晶[1] 刘可可[1] 张瑞永[1] 李红燕[1] 谭建锡[1] 彭安安[1] 聂珍媛[1] 

机构地区：[1]中南大学资源加工与生物工程学院,生物冶金教育部重点实验室,湖南长沙410083

出　　处：《现代生物医学进展》2009年第17期3208-3211,3250,共5页Progress in Modern Biomedicine

基　　金：国家自然基金创新研究群体科学基金项目(50621063);国家自然科学基金项目(50674101)

摘　　要：目的:对粘质沙雷氏菌(Serratia marcescens) XJ-01几丁质酶合成条件优化。方法:研究了碳源、氮源、温度、pH等单个因素对该菌几丁质酶合成的影响,并通过正交实验确定了该菌的最适酶合成条件。结果:该菌几丁质酶最优合成条件为:胶体几丁质5g/L、硫酸铵5g/L、培养温度32℃、最适pH8。结论:优化了S.marcescens XJ-01几丁质酶的合成条件。Objective： To determine the optimal conditions for chitinase production by Serratia marcescens XJ-01. Methods： The effects of culture conditions （e.g. carbon source, nitrogen source, temperature and pH） for chitinase production by Serratia marcescens XJ-01 were investigated by orthogonal experiment. Results： show that the optimal condition for producing chitinase is as follows： temper-ature of 32℃, pH 8.0, 5 g/L （NH4）2SO4 as the nitrogen source and 5 g/L colloidal chitin as the carbon source. Conclusions： The optimal conditions for chitinase production by Serratia marcescens were determined.

关 键 词：粘质沙雷氏菌 几丁质酶 优化 

分 类 号：Q93-331[生物学—微生物学] Q939.5