自制双试剂测定血清胆红素的探讨  

Preparation of double-reagent for determining the serum bilirubin

作　　者：王凤学[1] 李树仁[1] 

机构地区：[1]遵义医学院附属医院检验科,遵义563003

出　　处：《陕西医学检验》1998年第4期9-11,共3页Shaanxi Journal of Medical Laboratory Sciences

摘　　要：目的：建立准确的自动生化分析测定血清胆红素的方法．方法：采用双试剂测定，以尿素、咖啡因及Triton－X100组成的加速剂作为第一试剂，重氮试剂为第二试剂测定总胆红素。以对氨基苯磺酸溶液为第一试剂，亚硝酸钠浓度为1．45mmol／L的重氮试剂为第二试剂测定直接胆红素。结果：总胆红素回收率为99．85％，批内CV为0．82％，批间CV为1．54％，与咖啡因法比较r＝0．9999。t＝1．2323，P＞0．2，直接胆红素与改良Doumas法比较，r＝0．9986，t＝1．2508，P＞0．2，批内CV为1．20％，批间CV为1．91％，脂肪乳糜等对本法无干扰，0．5g／LHb有4％的负干扰。试剂至少稳定三个月．结论：方法结果准确，试剂稳定，可作为常规方法使用．objertive To establish accurate automated analysis procedure for determining the serum bilirubinMethods Urea .caffeine and Trion - X 100 were used as the first reagent and diazo p - amino - benxene -sulphonic acid anhy as the second reagent for determining the total bilirubin. To measure the direct bilirubin, the p -aniobenxene -sulphonic acid anhy was used as the first reagent and 1. 45 mmol/L sodium nitrite diazo - p-aminobenxene-sulphonic acid anhy as the second reagent. Results The total bilirubin concentrations measured by this procedure. (Y) in 90 sera correlated well with those results obtained with the modify Jendrassik-Grof(X), Y=1. 144X- 3.83,r= 0. 999 9. Within-run was 0. 82% and between -run was 1. 54% respectively. The calibration curves was linear in range of 0 to 513 umol/L. The recovery rate was between 99. 7 and 101. 0%. The direct bilirubin results was correlated well with the modify Doumas' (x ) Y= 0. 982X+0. 25,r=0.998 6 (n= 90). Within -run was 1. 20% and between-run was 1. 91 % respectively. The common interference were not effected, but bemoglobn pede a reduce effect. The reagents were stable for three months. Conclusions the results determinate by this procedure were accurate and the reagents were stable. It may be used for routine method.

关 键 词：双试剂 自动分析 总胆红素 直接胆红素 

分 类 号：R446.112[医药卫生—诊断学]