用于尿酶测定的液性质控物的制备  

Preparing a stable liquid control material for urine enzyme assays

作　　者：段勇[1] 王玉明[1] 张秋霞[1] 段永寿[1] 

机构地区：[1]昆明医学院第一附属医院,昆明650032

出　　处：《陕西医学检验》1998年第4期41-42,共2页Shaanxi Journal of Medical Laboratory Sciences

摘　　要：目的：研制一种用于尿酶试验的稳定液性质控物。方法：在离心后和pH调节为7．0的尿液中加入30％（V／V）的稳定剂和重蒸水30％（V／V），配制成尿酶的液性质控物。结果：经为期180d的试验表明，在4℃和-20℃的条件下，质控物中的β-N-乙酰葡萄糖苷酶、β-D-半乳糖苷酶、碱性磷酸酶和谷氨酰基转移酶的活性稳定。结论：该质控物能用于室内质量控制。Preparing a stable liquid control material for urine enzyme assays. A liquid control material for urine enzyme assays was prepared by addition of new stabilizer (30% ) and dH2O(30% ) to 7. 0. Tested for 180 days,the control material exhibited stable P-N-acetylglucosaminidase, β-D-galactosidase,alkaline phosphotase and gamma -glutamyltransferase at both 4 and -20℃. The control material was satisfactory for being used as internal quality control in urine enzynle assays.

关 键 词：质控物 制备 碱性磷酸酶 谷氨酰基转移酶 

分 类 号：R446[医药卫生—诊断学]