大鼠肠道缺血再灌注损伤时高迁移率族蛋白1变化及淋巴引流对肠屏障的保护作用  

Change of high mobility group box 1 and effect of drainage of intestine lymph fluid on rat gut barrier dur- ing intestine ischemia-reperfusion

作　　者：陈雪峰[1] 何桂珍[1] 董良广[1] 崔晓雨[1] 舒红[1] 王秀荣[1] 范东梅 

机构地区：[1]中国医学科学院北京协和医学院北京协和医院肠外肠内营养科,100730 [2]山东省莱阳市人民医院内科

出　　处：《中华临床营养杂志》2009年第2期91-94,I0001,共5页Chinese Journal of Clinical Nutrition

基　　金：基金项目：国家自然科学基金（30471707）

摘　　要：目的研究大鼠肠道缺血再灌注损伤时，内源性配体高迁移率族蛋白1（HMGB1）的变化及淋巴引流对肠屏障损伤的影响。方法32只健康雄性SPF级sD大鼠随机分为空白组、假手术组、肠道缺血再灌注组（I／R）和肠道缺血再灌注＋淋巴液引流组（I／R＋引流组），每组8只。在缺血再灌注损伤后检测肠道损伤程度、HMGB1变化、内毒素移位情况及循环细胞因子。结果I／R组和I／R＋引流组大鼠的肠道损伤程度、内毒素水平、HMGB1和各炎症因子水平均显著高于空白组和假手术组（P〈0．05）；I／R组内毒素水平和各炎症因子显著高于I／R＋引流组（P〈0．05）。免疫组织化学染色结果显示，I／R组的HMGB1表达显著高于I／R＋引流组。结论缺血再灌注损伤可导致大鼠体内HMGB1水平升高和肠黏膜屏障损伤，HMGBl可能增加肠屏障损伤和系统炎症反应。肠淋巴引流能阻断肠-淋巴途径，改善肠屏障形态和功能，减少循环中炎症因子和内毒素水平。Objective To investigate the change of high mobility group box 1 （HMGB1） after intestine ischemia reperfusion （I/R） in rats, compare the effect of drainage of intestine lymph fluid on gut barrier, and ex- plore the possible mechanism of ischemia-reperfusion injury. Methods Thirty-two Sprague-Dawley （SD） rats （ SPF grade） were randomly divided into 4 groups with 8 rats in each group ： blank group, sham group, intestine is- chemia-reperfusion （I/R） group, and intestine ischemia-reperfusion with drainage of intestine lymph fluid （I/R ＋ drainage） group. Indicators of gut barrier function damage, translocation of endotoxin, and change of HMGB1 and cytokines were detected after intestine ischemia-reperfusion injury. Results The gut barrier function damage and levels of endotoxin, HMGB1, tumour necrosis factor-alpha （TNF-a）, interleukin-6 （IL-6）, interleukin-1 beta （ IL-113）, and soluble intercellular adhesion molecule-1 （ sICAM-1 ） were significantly lower in blank group and sham group than in I/R group and I/R ＋ drainage group （ P 〈 0.05 ）. Compared with the intestine I/R ＋ drainage group, the levels of endotoxin and cytokines were significantly higher in the intestine I/R group. The level of HMGB 1 was slightly higher than that in the intestine I/R ＋ drainage group, but such difference was not statistically significant （P 〉 0.05 ）. Immunohistochemical staining also revealed that the expression of HMGB1 was significant- ly higher in I/R group than in I/R ＋ drainage group. Conclusions Intestine isehemia-reperfusion injury can lead to the injury of intestine mucosal barrier and increase HMGB1 level. HMGB1 may deteriorate gut barrier function and increase the levels of systemic cytokines. Drainage of lymph fluid can block the gut-lymph pathway and thus decrease the levels of endotoxin and cytokines in systemic circulation and attenuate intestine ischemia-reperfusion injury.

关 键 词：肠道缺血再灌注损伤 高迁移率族蛋白1 肠黏膜屏障 肠淋巴液引流 

分 类 号：R57[医药卫生—消化系统]