HPLC法测定血浆中阿糖胞苷及阿糖尿苷浓度  

Determination of cytarabine and uracil arabinoside in plasma by HPLC

作　　者：王涛[1] 吕冬梅[1] 邱述玲[1] 翟小翠[2] 

机构地区：[1]徐州医学院附属医院,徐州221002 [2]徐州医学院药学院,2005级本科生徐州221002

出　　处：《药学与临床研究》2009年第5期435-437,共3页Pharmaceutical and Clinical Research

基　　金：江苏省临床药学研究基金(编号:L07014)

摘　　要：目的：建立同时测定血浆中阿糖胞苷和阿糖尿苷浓度的高效液相色谱法，并用于1例急性非淋巴细胞白血病患儿的血药浓度监测。方法：采用反相高效液相色谱法，色谱柱：XTerra C18（4．6mm×250mm，5μm）；流动相：0．01mol·L^-1的磷酸盐缓冲液（pH=6．0）；检测波长：280nm；流速：0．95mL·min^-1；柱温：30℃。结果：阿糖胞苷血药浓度在0．25～21．74mg·L^-1范围内线性关系良好（r=0．9996），检测限为0．1mg·L^-1，日内、日间RSD小于5．0％和8．0％；阿糖尿苷血药浓度在0．99～86．96mg·L^-1范围内线性关系良好（r=0．9993），检测限为0．4mg·L^-1，日内、日间RSD小于5．0％和8．0％。阿糖胞苷及阿糖尿苷平均提取回收率高于96．0％：结论：本方法简单、快速、准确。适用于临床上阿糖胞苷和阿糖尿苷的血药浓度监测及药动学研究．Objective： To establish a method for the determination of cytarabine （Ara-C） and uracil arabinoside （Ara-U） in plasma simultaneously by high liquid performance chromatography, and apply it to an acute non-lymphocytic leukemia children. Methods： The assaying was performed on a 4.6 mm × 250 mm, 5 μm particle, XTerra C18 column, the mobile phase consisted of 0.01 mol.L^-1 phosphate buffer （pH =6.0）. The flow rate was 0.95 mL·min^-1, the detection wavelength of UV was 280 nm, and column temperature was 30℃. Results： The linear blood concentration range for Ara-C was0.25 - 21.74 mg· L^-1 （ r = 0.999 6） with the minimum detection at 0. 1 mg· L^ -1, the relative standard deviations of intra-day and inter-day were below 5.0% and 8.0%. The linear blood eoneentration range for Ara-U was 0.99 - 86.96 mg· L^ - 1 （ r = 0. 999 3 ） with the minimum detection at 0.4 mg· L^ - 1, the relative standard deviations of intra-day and inter-day were below 5.0% and 8.0%. The average recovery of Ara-C and Ara-U was more than 96.0%. Conclusions： The method is simple, fast, accurate and clinieally applieable to Ara-C and Ara-U plasma concentration monitoring and pharmacokinetic studies.

关 键 词：阿糖胞苷 阿糖尿苷 HPLC法 血药浓度 

分 类 号：R969.1[医药卫生—药理学]