抑制NO合酶上调大鼠血管α_1-肾上腺素受体和三磷酸肌醇受体  

Nitric Oxide Synthase Inhibition Upregulates the α 1 Adrenoceptor and Inositol Trisphosphate Receptor Binding in Rat Arteries

作　　者：符民桂[1] 杨军[1] 庞永政 唐朝枢[1] 刘乃奎[1] 

机构地区：[1]北京医科大学第一临床医学院心血管研究所

出　　处：《高血压杂志》1998年第4期291-295,共5页Chinese Journal of Hypertension

基　　金：国家自然基金

摘　　要：探讨ＮＯ系统和血管α１－肾上腺素受体α１－ＡＲ）及三磷酸肌醇受体（ＩＰ３Ｒ）系统在高血压发病中的相互作用。方法在常规饲食中加入Ｌ－ＮＡＭＥ喂饲大鼠１或４周制备大鼠高血压模型；应用放射性配基结合实验观察α１－ＡＲ及ＩＰ３Ｒ的变化。结果应用Ｌ－ＮＡＭＥ处理一周，大鼠动脉血压升高３０（２ｍｍＨｇ（Ｐ＜０．０５），血浆ＮＯｘ含量则下降２５％（Ｐ＜０．０５）。主动脉肌膜α１－ＡＲ及肌浆网ＩＰ３Ｒ密度分别增加１２％和４０％。Ｌ－ＮＡＭＥ处理４周，大鼠血压升高７５±８ｍｍＨｇ（Ｐ＜０．０１），血浆ＮＯｘ含量下降５０％（Ｐ＜０．０１），主动脉肌膜α１－ＡＲ及肌浆网ＩＰ３Ｒ密度分别较对照组高７３％和１３７％（Ｐ＜０．０１），此时尾动脉肌膜ＡＲ及肌浆网ＩＰ３Ｒ密度亦较对照组增加５５％和５６％（Ｐ＜０．０１）。结论提示长期抑制ＮＯＳ引起大鼠持续性高血压的同时，可致大鼠血管α１－ＡＲ及ＩＰ３Ｒ明显上调。Aim\ This study was designed to investigate the relationship of NO system and α 1 adrenoceptor and inositol trisphosphate receptor systems in hypertension.\ Methods\ The model of NOS inhibition induced rat hypertension was prepared by treating with L NAME for 1 or 4 weeks, and the binding of α 1 adrenoceptor and inositol trisphosphate receptor was determined by radioactive ligand binding assay.\ Results\ It was found that in the rats treated with L NAME for 1 week, the blood pressure was significantly increased by 30±2 mmHg( P <0.05) than that in control group, and the plasma Nox levels was decreased by 25%( P <0.05). Meanwhile, the density of α 1 adrenoceptor and inositol trisphosphate receptor in the aorta were significantly increased by 12% and 40% respectivly; no changes was found in the caudal artery. In the rats treated with L NAME for 4 weeks,the blood pressure was increased by 75±8 mmHg( P <0.01) than that in control group, and the plasma NOx levels was decreased by 50%( P <0.01). Meanwhile, the density of α 1 adrenoceptor and inositol trisphosphate receptor increase further more in the aorta, and the alteration was found in the caudal artery as well.\ Conclusion\ NOS inhibition upregulates the α 1 adrenoceptor and inositol trisphosphate receptor in rat arteries.

关 键 词：NO 高血压 EDRF α1-AR IP3R 

分 类 号：R544.1[医药卫生—心血管疾病]