禽呼肠病毒琼扩抗原和血清的制备  

Preparation of AGP Antigen and Serum of Avian reovirus

作　　者：赵光辉[1,2] 孙建宏[1] 韩正博[1] 刘景利[1] 冯新畅[2] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所,黑龙江哈尔滨150001 [2]东北农业大学动物医学学院,黑龙江哈尔滨150030

出　　处：《动物医学进展》2009年第9期45-49,共5页Progress In Veterinary Medicine

摘　　要：以禽呼肠病毒S1133毒株尿囊腔接种10日龄无特定病原体(SPF)鸡胚,无菌收集24 h^120 h的死胚尿囊液,加1 mL/L的甲醛灭活,浓缩制得琼扩抗原;用该抗原制成灭活苗两次免疫SPF鸡,无菌采血分离制备阳性血清;选用SPF鸡无菌采血分离制备阴性血清。经过效价测定制备16单位琼扩抗原,8单位阳性血清;取免后的SPF鸡血样4个20~2-5稀释分别与1单位~8单位琼扩抗原做琼扩试验,确定了应该用4单位或8单位琼扩抗原做工作抗原;并以血清中和试验为参照证明琼扩试验在血清抗体监测中的可行性。本研究成功制备了禽呼肠病毒琼扩抗原与血清,为用琼扩试验定量检测禽呼肠病毒感染禽和疫苗免疫禽的抗体奠定了基础。The ReoS1133 was inoculated into chorioallantoic chamber or allantoic cavity of 10-day-old embryonated chick eggs,allantoic fluid of embryos died in 48 h-120 h was collected, deactivated and concentrated to get AGP antigen; Killed avian reovirus vaccine made by the antigen was immunized into SPF fowl twice to get positive serum; Negative serum can be got from SPF fowl. 16-unit AGP antigen and 8-unit positive serum were obtained by titer determination. The 4 or 8-unit AGP antigens were used to be work antigen by doing AGP test with 4 serum samples diluted 2^0-2^-5 and 1-8 unit AGP antigen. Compared with SN test, AGP was proved to be feasible. The characterized results demonstrated that AGP antigen and serum of avian reovirus will be useful in serum antibody detection in infected or immunized fowl.

关 键 词：禽呼肠病毒 琼扩抗原 琼扩试验 血清中和试验 

分 类 号：S852.659.4[农业科学—基础兽医学]