微波辅助萃取与超声波萃取丹参中丹酚酸B的比较研究  

Comparative Study on Extraction of Salvianolic Acid B from Salvia miltiorrhiza Bge by Microwave-assisted Extraction and Ultrasonic Extraction

作　　者：王桂花[1] 周峰[1] 任中楠[1] 杨屹[1] 

机构地区：[1]北京化工大学理学院,北京100029

出　　处：《分析科学学报》2009年第5期559-562,共4页Journal of Analytical Science

基　　金：国家科技支撑计划子课题(No.2006BAI09B07-02)

摘　　要：本文对微波辅助萃取和超声波萃取丹参中丹酚酸B进行了比较研究,并用高效液相色谱法(HPLC)测定了丹参中丹酚酸B。考察了微波辅助萃取和超声波萃取参数的影响,在各自最佳萃取条件下进行了丹参中的丹酚酸B提取率的比对,结果表明:微波辅助萃取6 min的提取率高于超声波萃取30 min的提取率。微波辅助萃取法与超声波萃取法相比具有省时、高效和溶剂用量少的特点。利用指纹图谱比较了两种萃取方式提取的化学成分的差异,结果显示两种萃取方法提取的主要成分组成基本相同,其共有成分比例相近。Microwave-assisted extraction（MAE） and ultrasonic extraction were evaluated for the extraction of Salvianolic acid B from Salvia miltiorrhiza Bge in this work.The extracts were analyzed by high performance liquid chromatography（HPLC）.Various MAE and UE experimental conditions were investigated and the optimum conditions were obtained.The results showed that the percentage extraction of Salvianolic acid B from Salvia miltiorrhiza Bge by MAE is higher than that by UE at their optimum extraction conditions.Compared with UE, MAE was a rapid method with higher yield and less solvent consumption. Comparison of the fingerprints of the extracts from Salvia miltiorrhiza Bge by MAE and UE showed that the main chemical substances and their contents obtained by MAE and UE were similar.

关 键 词：微波辅助萃取 超声波萃取 丹参 丹酚酸B 高效液相色谱法 

分 类 号：O657.72[理学—分析化学]