二甲酚橙-KBrO_3体系阻抑反应动力学光度法测定蛋白质  

Determination of Trace BSA by Inhibition Kinetic Spectrophotometry

作　　者：孙晓艳[1] 李贤珍[1] 李建平[2] 

机构地区：[1]中国地质科学院郑州矿产综合利用研究所检测中心,河南郑州450006 [2]桂林工学院材料与化学工程系,广西桂林541004

出　　处：《分析科学学报》2009年第5期609-611,共3页Journal of Analytical Science

基　　金：广西教育厅科研项目(200508135)

摘　　要：在稀硫酸介质中,蛋白质可灵敏地阻抑溴酸钾氧化二甲酚橙褪色的兰多尔特（Landolt）反应,据此建立了阻抑动力学光度法测定痕量蛋白质的新方法。在优化实验条件下,于430 nm波长处进行测定,蛋白质含量在0.2-2.0 mg/L范围内与吸光度呈线性关系,检出限为0.141 mg/L,标准加入回收结果满意。In this paper,the discoloration of Xylenol orange by potassium bromate in H2SO4 medium-Landolt reaction was studied.Based on this fact,a new method for the determination of trace BSA was established by inhibition kinetic spectrophotometry.Under the optimum condition,the linear range is 0.2～2.0 μg/mL,and the detection limit of the method was 0.141 mg/L.The developed method was applied to the determination of BSA with satisfactory results.

关 键 词：蛋白质 阻抑反应 动力学光度法 兰多尔特反应 

分 类 号：O657.3[理学—分析化学]