中药大青叶有效单体抗流感病毒作用  

Antiviral Activity of the Effective Monomers from Folium Isatidis against Influenza Virus in Vitro

作　　者：刘钊[1] 杨占秋[2] 肖红[2] 

机构地区：[1]中南民族大学药学院,武汉430074 [2]武汉大学医学院病毒学研究所,武汉430071

出　　处：《中南民族大学学报（自然科学版）》2009年第3期42-46,共5页Journal of South-Central University for Nationalities：Natural Science Edition

基　　金：中南民族大学自然科学基金资助项目(YZQ05011)

摘　　要：采用中药大青叶有效单体进行体外抗流感病毒（H1N1）的研究,通过观察病毒引起的细胞病变效应（CPE）、MTT法检测细胞活性,红细胞凝集实验检测病毒血凝滴度,考核药物的抗病毒作用.结果表明：大青叶有效单体1#,2#,3#,4#对流感病毒无直接灭活作用,也不能阻止流感病毒的吸附,而能抑制流感病毒在MDCK细胞内的的生物合成.其半数抑制浓度（IC50）分别为30.80,29.74,27.56,25.85 mg/L,治疗指数（TI）分别为6.33,9.30,10.77,15.05.其抗流感病毒效果优于病毒唑（IC50=101.05 mg/L,TI=5.25）,和抗病毒口服液（IC50=104.41 mg/L,TI=5.03）,P〈0.01.在160 mg/L时能抑制流感病毒在MDCK细胞内的增殖达90%左右.在40-160 mg/L内大青叶1#,2#,3#,4#单体能明显降低流感病毒血凝滴度（P〈0.05）.大青叶有效单体1#,2#,3#,4#能安全高效地抑制流感病毒在MDCK细胞中的增殖.The antiviral activity of the 4 effective monomers from Chinese medicinal herb Folium Isatidis（FI）1, 2, 3 and 4 against influenza virus （H1N1） was invesitingated by cell culture technigue to observe cytopathic effect （CPE）, MTT colorimetric assay and hemagglutination titer test to obtain virus inhibitory rate. The results showed that the four monomers may inhibit influenza virus biological synthesis other than directly inactivate this virus or block its adsorption onto susceptible cells. The inhibitory concentration （IC50） of the four monomers were 30. 80, 29.74, 27.56, 25.85 rag/L, respectively and the treatment indexes（TI） were figured out as 6.33,9.30,10. 77 and 15.05. Their antiviral activities are more effective than ribavirin （IC50= 101.05 rag/L, TI= 5.25） and antiviral liquor（IC50 = 104. 41 mg/L, TI = 5.03） at the same dosage level （P〈0. 01）. 160 mg/L of the monomers can nearly inhibit 90 percents of the proliferation of influenza virus in MDCK cells. Moreover, in the range of 40-160 mg/L the four monomers can significantly decrease the hemagglutinin titer of influenza virus （P〈0.05）. we thus conclude that the monomers No. 1, 2, 3, 4 from Folium Isatidis can inhibit the proliferation of the influenza virus in MDCK cell effectively and safely.

关 键 词：大青叶 单体 流感病毒 抗病毒作用 

分 类 号：Q949.748.3[生物学—植物学] Q291