绵羊和山羊BMP15基因FecX^R突变的检测  

Detection of the FecX^R Mutation of BMP15 Gene in Sheep and Goats

作　　者：狄冉[1] 冯涛[2] 储明星[1] 张英杰[2] 方丽[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所农业部畜禽遗传资源与利用重点开放实验室,北京100193 [2]甘肃农业大学动物科学技术学院,兰州730070

出　　处：《畜牧兽医学报》2009年第9期1303-1307,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家863计划(2006AA10Z139);国家973计划(2006CB102105);国家科技支撑计划(2008BADB2B01;2006BAD01A11;2006BAD13B08);国家自然科学基金项目(30540052);北京市自然科学基金项目(6062023);中国农业科学院北京畜牧兽医研究所基本科研业务费专项(ywf-rc-1)资助

摘　　要：为了检测骨形态发生蛋白15(BMP15)基因在绵羊和山羊中的突变,本研究采用PCR-SSCP方法在高繁殖力绵羊品种(小尾寒羊和湖羊)、山羊品种(济宁青山羊和波尔山羊)和低繁殖力绵羊品种(多赛特和特克塞尔)、山羊品种(内蒙古绒山羊和安哥拉山羊)中检测BMP15基因FecXR突变,并对小尾寒羊和济宁青山羊扩增片段进行核苷酸和氨基酸序列分析。结果表明,这8个羊品种均没有发生与Rasa Aragonesa绵羊相同的FecXR突变;小尾寒羊核苷酸序列与GenBank中绵羊BMP15序列(AF236079,NM_001114767)完全一致,济宁青山羊与小尾寒羊相比存在3处碱基不同(T529G、C530G和T576C)和2个氨基酸差异(V155G和S171P)。可见BMP15基因影响Ra-sa Aragonesa绵羊高繁殖力的突变FecXR对小尾寒羊、湖羊、济宁青山羊和波尔山羊的繁殖力均没有显著影响。To detect the mutations of bone morphogenetic protein 15（BMP15） gene in both high prolificacy breeds（Small Tail Han sheep,Hu sheep,Jining Grey goat and Boer goat） and low prolificacy breeds（Dorset sheep,Texel sheep,Inner Mongolia Cashmere goat and Angora goat）,the method of PCR-SSCP was used.Both the nucleotide sequences and the amino acid sequences were compared in the amplification fragments of both Small Tail Han sheep and Jining Grey goat.The results indicated that none of the four sheep and the four goat breeds carried the same FecX^R mutation of the BMP15 gene as Rasa Aragonesa sheep.The nucleotide sequence of Small Tail Han sheep was completely identical with that of the sheep BMP15 sequence（AF236079,NM001114767） in the GenBank.Three base substitutes（T529G,C530G and T576C） and two amino acid changes（V155G and S171P） were found in Jining Grey goat compared with Small Tail Han sheep.The FecX^R mutation of the BMP15 gene had no significant effect on high prolificacy of Small Tail Han sheep,Hu sheep,Jining Grey goat and Boer goat.

关 键 词：绵羊 山羊 繁殖力 骨形态发生蛋白15基因 FecXR突变 PCR-SSCP 

分 类 号：S826[农业科学—畜牧学] S813.8[农业科学—畜牧兽医]