中国部分地区猪圆环病毒2型的基因型分析  

Survey on Molecular Epidemiology of Porcine Circovirus Type 2 Isolates Collected from Some Area of China with a PCR

作　　者：李文洁[1] 李文涛[1] 严伟东[1] 陈焕春[1] 何启盖[1] 

机构地区：[1]华中农业大学动物医学院农业微生物学国家重点实验室,武汉430070

出　　处：《畜牧兽医学报》2009年第9期1358-1362,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家公益性行业(农业)科研专项(nyhyzx07-034);现代农业产业技术体系专项(nycytx-009)

摘　　要：对中国11个省(市)部分猪场收集的812份病料,应用ORF2-PCR进行检测,发现有235份为PCV2阳性病料。利用鉴别PCR方法从235份阳性病料中,检出10份PCV2a基因型和133份PCV2b基因型,检出率分别为4.2%、56.6%。进一步对ORF2-PCR检测阳性的部分模板进行全基因组扩增,构建阳性重组质粒,对插入质粒的片段进行测序鉴定,利用DNAStar进行同源性分析,同GenBank参考毒株绘制系统发育树,从系统发育树中也可分出PCV2a、PCV2b 2种基因型。鉴别PCR和测序结果说明,我国部分地区流行的猪圆环病毒2型以PCV2b基因型为主,少数为PCV2a基因型,也有既非PCV2a又非PCV2b的基因型(PCV2c?)。Tissue samples were collected from pig farms of different regions of 11 provinces in China.812 suspicious samples were detected by ORF2-PCR,and 235 samples were PCV2-positive.The genotype of 235 ORF2 fragments amplified from positive samples was identified by distinction PCR for PCV2a and PCV2b.The results showed that the isolated rates of PCV2a genotype and PCV2b genotype were 4.2% and 56.6%,respectively.A pair of primers was designed according to documented data in the GenBank,and the complete genomes of 235 PCV2-positive template were amplified by PCR.The PCR products of complete genome of PCV2 isolates were cloned and sequenced.The sequences were compared with those of other PCV2 strains in the GenBank,the results indicated that genomic sequences of PCV2 strains tested in this study could be divided into three genotypes,PCV2a,PCV2b and the other genotype that neither PCV2a nor PCV2b（PCV2c？）.The results mentioned above also revealed that the epidemic characteristics of genotype of PCV2 in some areas of China were PCV2b,secondly for little PCV2a as well as neither PCV2a nor PCV2b（PCV2c？）.

关 键 词：猪圆环病毒2型 分子流行病学 PCV2a PCV2b 

分 类 号：S852.659.2[农业科学—基础兽医学]