表皮生长因子及其受体在山羊胎儿皮肤发育中的表达特征  

Expression Characteristics of EGF and EGFR in Developing Skin of Goat Fetus

作　　者：卿素珠[1] 林吉茂[1] 张灵枝[1] 王磊磊[1] 罗时双[1] 

机构地区：[1]西北农林科技大学动物医学院,杨凌712100

出　　处：《畜牧兽医学报》2009年第9期1383-1388,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：陕西省自然科学基金资助项目(2004C120);西北农林科技大学人才基金资助项目

摘　　要：运用组织学和免疫组化方法对山羊皮肤发育的组织学特点、山羊皮肤发育过程中表皮生长因子(EGF)及其受体(EGFR)的分布及变化规律进行了系统研究。结果显示:(1)表皮结构形成于胎儿发育的6周龄左右,开始为单层上皮,而后逐渐增殖为复层、厚度不断增加,15周龄后逐渐变薄;真皮层在10周龄时形成,11周龄后陆续有皮肤衍生结构从中发育形成。随着胚胎的继续发育,皮肤各部分结构的发育渐趋完善。(2)在胎儿发育的第6周就出现EGF与EGFR的较弱表达,以后随着胎龄的增加,EGF与EGFR表达量逐渐增加。从表达分布看,在胎儿发育的11周前,EGF阳性反应主要定位于表皮基底层细胞、毛囊上皮细胞和真皮成纤维细胞等的胞质内,EGFR则主要位于相应细胞的膜上;11-16周,EGF和EGFR表达量逐渐增高,分布范围从表皮的基底层细胞、棘细胞、毛囊上皮细胞和成纤维细胞扩展到血管内皮细胞、汗腺上皮细胞和竖毛肌,EGF主要定位于这些细胞的胞质,EG-FR则主要分布于细胞膜;17周至出生,随着表皮的明显变薄,EGF及EGFR主要分布于表皮基底层细胞和毛囊上皮细胞,阳性反应继续增强。皮肤发育期间,EGF及EGFR的表达量总体呈增长趋势,只是EGFR的表达相对滞后,两者的表达曲线一致、相关性极显著。表明EGF及EGFR在皮肤及其衍生结构的发育中发挥着重要作用。The histological characteristics,distribution and variation discipline of epidermal growth factor（EGF） and epidermal growth factor receptor（EGFR） during skin′s development of goat fetus were studied with histological methods and immunohistochemistry.The results showed that the epidermis formed at the embryonic 6th week,and then the thickness increased gradually because of transition from simple epithelium to stratified epithelium,and decreased after the 15th week.The structure of dermis occurred at the 10th week,and the derivates in dermis appeared after the 11th week.As the process of prenatal development,all structures of skin matured gradually.Low expression of EGF and EGFR could be observed at the 6th week.Afterwards,the expression of EGF and EGFR increased as growth.Before the 11th week,EGF positive reaction mainly located in cytoplasm of basal layer cells of epidermis,hair follicular epithelia cells and fibroblasts,and EGFR mainly located on cell membrane of these cells.From 11th to 16th week,the expressional range of EGF and EGFR extended from basal layer cells,prickle cells,hair follicular epithelia cells and fibroblasts to vascular endothelial cells,epithelial cells of sweat gland and arrector pili muscle.EGF mainly distributed in cytoplasm of these cells,and EGFR were also located on membrane of these cells.From 17th week to birth,EGF mainly located in basal layer cells and hair follicular epithelia cells as the skin thinned,the positive intensity continued to increase.The expressed amount of EGF and EGFR trended to increase during the whole development stage just accompanied by a hysteresis in expression of EGFR.There were extreme significant correlations between expressed amount of EGF and EGFR.The results demonstrated that EGF and EGFR played important roles in the development of skin and its derivates.

关 键 词：表皮生长因子 表皮生长因子受体 皮肤 发育 山羊 

分 类 号：S852.16[农业科学—基础兽医学]