水牛卵丘颗粒细胞凋亡与卵母细胞体外发育潜能的研究  

Study on the Relationship between Cumulus Granulose Cells Apoptosis and Oocyte Development in Buffalo

作　　者：王晓丽[1] 杨娟[1] 陈玉[1] 成俊萍[1] 潘红平[1] 韦精卫[1] 陆凤花[1] 石德顺[1] 

机构地区：[1]广西大学动物科学技术学院,南宁530005

出　　处：《畜牧兽医学报》2009年第9期1400-1404,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家"863计划"项目(2007AA100505);国家自然科学基金资助项目(30469090);广西大学博士启动基金(X071099)

摘　　要：为了探讨水牛卵母细胞体外发育潜能与卵丘颗粒细胞凋亡之间的关系,本研究将水牛卵母细胞体外培养22-24 h后,取其卵丘颗粒细胞,采用Annexin V-FITC法进行颗粒细胞凋亡染色,进行凋亡率的分析,并与卵母细胞的成熟情况和卵母细胞的评分及形成卵裂、囊胚的结果进行对照。结果发现,随着卵母细胞成熟时间的增加,卵丘颗粒细胞凋亡率升高;随着卵母细胞评分和发育潜能的降低,卵丘颗粒细胞凋亡率逐渐升高。由此表明,卵母细胞发育潜能与卵丘颗粒细胞凋亡密切相关,通过测定卵丘颗粒细胞的凋亡率可预测未成熟卵母细胞的发育潜能。The relationship between the developmental ability of buffalo oocytes and the apoptosis of granulose cells was investigated in this study.Granulose cells were analyzed on the apoptosis by Annexin V-FITC after the buffalo oocytes were cultured 22-24 h in vitro,and which was compared with the maturity and the scoring of oocyte as well as the rate of cleavage and embryos.Results showed that the rate of apoptosis increased with the maturity of oocyte.While with the scoring and developmental capacity of the oocyte decreasing,the rate of apoptosis of granulose cells was increased.The results indicated that the relationship between the developmental capacity of the oocyte and apoptosis of granulose cells was significant.The apoptosis rate of granulose cells could be a developmental potential predictor of the immature oocytes.

关 键 词：卵丘颗粒细胞 凋亡 卵母细胞 体外成熟 

分 类 号：S823.8[农业科学—畜牧学] S814.1[农业科学—畜牧兽医]