牛肺泡巨噬细胞微小RNA的克隆与鉴定  

Cloning and Identification of MicroRNA from Bovine Alveolar Macrophage

作　　者：徐广贤[1] 张艳[1] 贾浩[1] 周萍[1] 王玉炯[1] 

机构地区：[1]宁夏大学生命科学学院,银川750021

出　　处：《畜牧兽医学报》2009年第9期1410-1413,共4页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家重点基础研究发展计划(973)项目子课题(2006CB504401);国家自然基金项目(30860207;30960275);高等学校科技创新工程重大项目培育资金项目(706057);宁夏高等学校科研项目;宁夏大学科研基金项目(ZR200724)

摘　　要：MicroRNA（mi RNA）是一类长19-26个核苷酸的内源性非编码单链小分子RNA,其异常表达将会导致生理的异常和疾病的产生。为了研究mi RNAs在牛肺泡巨噬细胞抗菌机制中的作用,构建了牛肺泡巨噬细胞mi RNAcDNA文库,从438个克隆中筛选出22个mi RNAs,其中有19个mi RNAs在牛的mi RBase数据库中没有发现,但是有8个mi RNAs与人和（或）鼠中的序列完全同源,因此,将它们命名为Bta-mi R-141、Bta-mi R-187、Bta-mi R-191、Bta-mi R-448、Bta-mi R-589、Bta-mi R-873、Bta-mi R-463和Bta-mi R-562;另外有11个mi RNAs在所有mi R-Base数据库中都没有发现,有待进一步研究。这些数据为研究mi RNAs调控牛肺泡巨噬细胞抗菌机制奠定了基础。MicroRNAs （miRNAs） are a class of non-coding single-stranded RNA that play vital roles in many biological processes by regulating the expression of genes. To study the antibacterial mechanism of microRNAs in bovine alveolar macrophage, a cDNA library of microRNAs was constructed. By sequencing, 22 microRNAs have been screened in 438 clones. There are 19 microRNAs which have not been reported in miRBase, and 8 of them were sequence homology with human and rat. They are respectively named Bta-miR-141, Bta-miR-187, Bta-miR-191, Bta-miR- 448, Bta-miR-589, Bta-miR-873, Bta-miR-463 and Bta-miR-562 by the rules. 11 others have not been found in any miRBase and should be further studied.

关 键 词：MICRORNA 牛肺泡巨噬细胞 克隆 鉴定 

分 类 号：S852.2[农业科学—基础兽医学] Q522[农业科学—兽医学]