细粒棘球蚴囊液对肝细胞表面TLR4表达的调节  

The expression of TLR4 in liver cell treated with cyst fluid of Echinococcus granulosus

作　　者：张亚楼[1] 黄河[1] 方全华[1] 刘涛[1] 任智慧[1] 李亮[1] 温浩[1] 

机构地区：[1]新疆医科大学第一附属医院医学研究中心,乌鲁木齐830054

出　　处：《中华微生物学和免疫学杂志》2009年第9期858-860,共3页Chinese Journal of Microbiology and Immunology

基　　金：新疆医科大学第一附属医院科研奖励基金（2007-YFY-04）;国家自然科学基金（30760228）

摘　　要：目的通过研究细粒棘球绦虫囊液对人肝细胞中Toll样受体（TLR）4表达的影响，探讨TLR在启动囊液抗细粒棘球绦虫信号转导通路中的作用。方法用定量PCR检测肝细胞7404囊液处理前后TLR4和TGF-β1的表达变化。结果肝细胞7404经囊液处理后随剂量增加TLR4表达减弱，TGF-β1随剂量增加表达增强，TLR2表达在各组均很低。结论囊液可以上调TLR4的表达，提示囊液诱导的抗细粒棘球绦虫的信号转导途径可能由TLR4引起。高剂量囊液有助于TGF—β1所致的免疫逃避作用。Objective To explore the signal transduction pathway of cyst fluid of Echinococcus granulosus in anti-parasite mechanisms through investigating the effect of cyst fluid on the expression of Toll- like reeeptor（TLR） in cells. Methods Changes of TLR4 and TGF-β1 expression of 7404 liver cells were detected by quantitative PCR. Results After treatment with increasing concentration cyst fluid the expression of TLR4 was reduced. TGF-β1 expression of liver cells increased with the dose. TLR2 expressions in each group were very low. Conclusion Cyst fluid can increase the expression of TLR4, suggesting that the TLR4 signal transduction pathway involve anti-cyst fluid of Echinococcus granulosus. High concentrations of cyst fluid contribute to TGF-β1 expression which plays a role in immune evasion.

关 键 词：细粒棘球绦虫 囊液 TOLL样受体 天然免疫 

分 类 号：R392[医药卫生—免疫学]