珍禽霉形体抗体检测方法的建立和应用  

DEVELOPMENT AND APPLICATION OF THE METHODS TO DETECT MYCOPLASMA ANTIBODY FROM ZOO RARE BIRDS

作　　者：孙建和[1] 严亚贤[2] 张琼[3] 汤锡珊[3] 

机构地区：[1]上海农学院生物技术研究所,上海201101 [2]上海农学院动物科学系,上海201101 [3]上海动物园,上海200335

出　　处：《上海农学院学报》1998年第3期213-217,226,共6页

摘　　要：本试验经培养特性、形态特征、红细胞吸附试验和生长抑制试验等从8株珍禽霉形体中筛选出1株具有代表性的菌株MG—P,将MG—P和标准株MG-S分别建立了HI和Dot-ELISA试验,检测3批120份共 100多种珍禽动物的血清样本,MG—P的阳性检出率为HI 14. 2％（17/20）,Dot—ELISA 16.7％（20/120）,两法阳性符合率为 85％（17/20）;MG—S_6的阳性检出率为HI 9.2％（11/120）,Dot—ELISA16. 7％（20/120）,两法符合率为 55％（11/20）。以MG—P为检测抗原,将 HI、Dot—ELISA和常规血清平板凝集试验（SPA）比较,结果表明HI具有高特异性和敏感性,而Dot—ELISA和SPA具有较高的非特异性,且 SPA试验重复性差。因此以 MG—P建立的HI试验为检测珍禽霉形体血清抗体的最佳方法。One strain mycoplasma (MG-P) was selected from eight strains isolated from zoo rare birds by culture property, morphological character, red cell adhesion and growth inhibition. MG-P and MG - Ss were propagated in selected medium and then hyperimmune sera were prepared in poultry. The methods of HI and Dot - ELISA with the two antigens for detection of mycoplasma antibodies were developed and applied in 3 batches 120 samples from zoo poultry. The positive rate with MG-P was 14.2% by HI and 16.7% by Dot-ELISA, while with MG-& were 9.2% and 16.7%, respeceively.The test of sensitivity, specificity and repetition of HI, Dot - ELISA and serum - plate agglutination (SPA) showed that HI had higher specificity and sensitivity, Dot -ELISA and SPA had higher no - specificity and SPA also had lower repetition. According to those results the HI with MG - P was the best method to detect mycoplasma antibody from zoo rarebirds.

关 键 词：霉形体 抗体 HI DOT-ELISA SPA 

分 类 号：S858.924.3[农业科学—临床兽医学]