蜡质芽孢杆菌超氧化物歧化酶(SOD)的纯化研究  

Purification of Superoxide Dismutase (SOD) from Bacillus cereus

作　　者：苏宁[1,2,3] 王桂峰[1,2,3] 徐维敏 衣文平 王琦[1,2,3] 梅汝鸿 

机构地区：[1]中国农业科学院生物中心 [2]山东省农业厅优质农产品中心 [3]中国农业大学植物科技学院植保系

出　　处：《农业生物技术学报》1998年第3期235-238,共4页Journal of Agricultural Biotechnology

摘　　要：蜡质芽孢杆菌（Ｂａｃｉｌｕｓｃｅｒｅｕｓ）经培养后，离心收集菌体，将菌体破碎、离心、经（ＮＨ４）２ＳＯ４分级沉淀、ＳｅｐｈａｄｅｘＧ－７５凝胶过滤和ＤＥＡＥ－５２柱层析所获得的超氧化物歧化酶进行ＳＤＳ－ＰＡＧＥ电泳为一条带，亚基分子量为１７７８３Ｄ，表明制备得到了电泳纯品。将该样品经氰化钾、双氧水、氯仿－乙醇抑制实验和特征吸收光谱分析，鉴定出此酶为Ｆｅ－ＳＯＤ，比活为：１７５３．８ｕ。After fermenting, SOD from Bacillus cereus was purified by a stepwise procedure consisting of (NH 4) 2SO 4 fractionation, sephadex G 75 gel filtration and DEAE 52 cellulose chromatography. This procedure resulted in a single band on sodium dodecyl sulfate polyacrylamide gels indicating that the preparation was homogeneous. The subunit molecular weight of this protein was estimated at 17 783 daltons. The nature analysis by the spectrum of ultraviolet and the inhibition of cyanide, H 2O 2 and chloroform ethanol indicated that the pure preparation was Fe SOD. The relative activity was 1753.8u.

关 键 词：蜡质芽孢杆菌 超氧化物歧化酶 纯化 

分 类 号：Q939.124[生物学—微生物学] Q554.9