传染性支气管炎病毒S1纤突糖蛋白基因的克隆及部分序列分析  

Cloning and Sequencing of the S1 Spike Protein Gene of the Infectious Bronchitis Virus

作　　者：陈丽君[1,2] 王英[1,2] 胡建华[1,2] 周宗清 王建荣[1,2] 

机构地区：[1]上海农业科学院畜牧兽医研究所 [2]上海市农业遗传育种重点实验室动物遗传工程研究室

出　　处：《农业生物技术学报》1998年第3期239-243,共5页Journal of Agricultural Biotechnology

摘　　要：大纤突Ｓ糖蛋白是传染性支气管炎病毒重要的免疫相关性蛋白之一，参考已发表的ＩＢＶ－Ｂｅａｕｄｅｔｅ株Ｓ１基因ＤＮＡ序列，合成一对特异性引物，以ＩＢＶ－ＲＮＡ为模板，应用ＲＴ－ＰＣＲ方法，获得传染性支气管炎病毒上海分离株Ｓ２Ｔ２－Ｓ１纤突糖蛋白基因，长度１．６５ｋｂ，利用引物设计中的ＢａｍＨⅠ和ＨｉｎｄⅢ位点将其克隆到载体ｐＳＫ（＋）中。对该基因进行限制性酶切分析，结果与已报道的相一致。通过Ｓ１基因的Ｃ端部分核苷酸序列和氨基酸分析及与Ｂｅａｕｄｅｔｅ株、日本分离株和Ｍ４１标准株相比较，结果表明该基因的核苷酸序列和氨基酸序列同源率分别为９５．１％，９３．６％和９６．７％以及９５．６％，９２．６％和９７．１％。S1 spike protein is one of the important functional proteins of infectious bronchitis virus(IBV). Based on the reported sequence of the spike protein gene, IBV Beaudette, two specific primers were synthesized. A 1652bp DNA fragment was amplicated by RT PCR from the viral RNA of IBV, Shanghai isolate S 2T 2, and further cloned into plasmid pSK(+). Restriction mapping revealed that the result was consistent with the reported data. Comparison of sequence of S1 spike gene of S 2T 2 with IBV Beaudette, Japan isolate, M41 showed that the homologies were 95.1% 、93.6% and 96.7% in amino acid respectively .

关 键 词：传染性支气管炎 病毒 S1糖蛋白基因 家畜 

分 类 号：S852.6[农业科学—基础兽医学]