兰州百合多糖硒酸酯的合成及表征  

Synthesis and Characterization of Seleno-Lilium davidii var. unicolor Salisb Polysaccharides

作　　者：张继[1] 刘忠旺[1] 王风霞[1] 杨玉萍[1] 田玉汝[1] 孙文秀[2,3] 王云普[2] 

机构地区：[1]西北师范大学生命科学学院,甘肃特色植物有效成分与制品工程技术研究中心,兰州730070 [2]甘肃省高分子材料重点实验室,兰州730070 [3]内蒙古农业大学食品科学与工程学院,呼和浩特010018

出　　处：《高分子通报》2009年第10期48-52,共5页Polymer Bulletin

基　　金：科技部支撑计划(2008BAK51B05);国家自然科学基金(50773064)

摘　　要：用水提取兰州百合块茎粗多糖,经酶和savage联用法脱蛋白质,得兰州百合多糖(LP)。在HNO3-OBaCl2催化作用下,利用亚硒酸钠水解产生的HO SeOH对兰州百合多糖的—OH进行修饰,首次合成了兰州百合多糖硒酸酯(seleno-LP)。通过正交实验得出最佳反应条件是硝酸体积浓度0.5%,70℃反应10h,ICP测得此时硒含量为0.784mg/g,并通过紫外、拉曼光谱和热分析分别对合成产物进行表征,最后得出硒在合成产物中是以Se O的形式存在。Lilium davidii var. unicolor Salisb raw polysaccharides were extracted from Lilium davidii var. unicolor Salisb by distilled water. The Lilium davidii var unicolor Salisb polysaccharides were isolated through deproteinization by papain and savage. Seleno-Lilium davidii var. unicolor Salisb polysaccharides（Seleno-LP） were prepared by employing sodium selenite which hydrolyze generation of HO-↑O=Se-OH to modify the LP under the catalysis of nitric acid-barium chloride. The favor reaction conditions were at 70℃ for 10 hours in the 0.5 % （v/v） nitric acid. The selenic content of seleno-polysaccharide determined by ICP-AES was 0. 784mg/g. The title products were characterized by Ultraviolet spectra, Laser-Raman spectrum and differential thermal analysis （DTA） and thermogravimetric analysis （TG）. We come to the conclusion that Se exist in the form of Se = O in the title products.

关 键 词：兰州百合多糖硒酸酯 合成 表征 

分 类 号：R284.1[医药卫生—中药学]