用PCR诊断鸡传染性鼻炎的进一步研究 Ⅰ.病料收集时间  

Futher Studies on the PCR Test for Diagnosis of Infectious Coryza I. Specimen Collection

作　　者：宋程[1] 陈小玲[1] 陈葵[1] 

机构地区：[1]北京市农林科学院畜牧兽医研究所

出　　处：《中国兽药杂志》1998年第4期1-3,共3页Chinese Journal of Veterinary Drug

摘　　要：研究鸡传染性鼻炎（ＩＣ）的ＰＣＲ临床诊断方法时，对人工感染鸡进行了ＰＣＲ样品收集时间的研究，并与几种常用的ＩＣ诊断方法进行了比较。结果表明，试验鸡在感染１５ｄ扑杀后，临床症状阳性的鸡数明显下降；眶下窦粘液检查和细菌分离比临床症状检查敏感；细菌分离易污染，尤其是活鸡取样；ＰＣＲ方法敏感、准确，但其检测能力也随病程的结束而下降。适宜的ＰＣＲ样品收集时间建议为感染后的１～３周内。The suitable specimen collection time from Infectious coryza (IC) fowls for diagnosis by PCR test and some other common methods were investigated in experimental infected chickens. The results showed that of more than 1/3 chickens the clinical signs disappeared at 15 (infected at 6 weeks old) or 6 days or 6 days (linfected at 14 weeks old) after infection. Bacterial examinations of speciemen from the living chickens always were confused by contaminators. The PCR test was sensitive and accurate, it should be performed within 1￣3 weeks of infection.

关 键 词：传染性鼻炎 鸡 PCR 病料收集时间 

分 类 号：S858.317.7[农业科学—临床兽医学]