羊骨髓来源的低分子活性物质对大鼠骨髓基质细胞成骨分化的作用  

Effects of the Active Substance with Low Molecular Weight from Goat Bone Marrow on the Osteogenic Differentiation of Bone Marrow Stromal Cells

作　　者：肖毅[1] 王建国[1] 白增亮[1] 

机构地区：[1]山东大学生命科学学院发育免疫学实验室,济南250100

出　　处：《中国中医骨伤科杂志》2009年第10期11-13,共3页Chinese Journal of Traditional Medical Traumatology & Orthopedics

基　　金：山东省自然科学基金项目资助(NO.11200004040740)

摘　　要：目的:观察羊骨髓来源的低分子活性物质诱导体外培养的大鼠骨髓基质细胞成骨分化的作用。方法:取新鲜的羊腿骨提取羊骨髓来源的低分子活性物质。取6周龄SD大鼠,密度梯度离心法分离、培养大鼠骨髓基质细胞,第三代大鼠骨髓基质细胞在羊骨髓来源的低分子活性物质的诱导培养下,检测大鼠骨髓基质细胞碱性磷酸酶活性。结果:羊骨髓来源的低分子活性物质可以显著提高大鼠骨髓基质细胞的碱性磷酸酶活性。结论:羊骨髓来源的低分子活性物质可以明显促进大鼠骨髓基质细胞的成骨分化。Objective：To investigate the effects of the active substance with low molecular weight from goat bone marrow on the osteogenic differentiation of cultured bone marrow stromal ceils （MSCs） of rats. Methods.. Prepare the active substance with low molecular weight from the bone marrow of goat legs. The SD rats （aged 6 weeks） were sacrificed and the bone MSCs were separated by density gradient centrifugation and then cultured. We detected the alkaline phosphatase activity of the third passage of bone MSCs cultured by the active substance. Results：The activity of alkaline phosphatase was significantly elevated. Conclusion：The active substance with low molecular weight from goat bone marrow can promote the bone MSCs to osteogenic differentiation.

关 键 词：骨髓基质细胞 成骨分化 羊骨髓来源的低分子活性物质 

分 类 号：Q952[生物学—动物学]