一枝蒿总黄酮体外抗乙肝病毒作用机理的实验研究  

The Study on the Mechanism of Anti-hepatitis B Virus in vitro by Flavonoids Extract from Milfiol Herb

作　　者：郭姗姗[1] 高英杰[1] 时宇静[1] 苏丹[1] 崔晓兰[1] 王意忠[2] 

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]北京大学航天临床医学院,北京100049

出　　处：《中国实验方剂学杂志》2009年第10期72-74,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金面上项目(30672646);中国中医科学院自主选题项目(ZZ2006101)

摘　　要：目的:以HepG2.2.15细胞为乙肝病毒感染的体外实验模型,观察一枝蒿总黄酮对核转录因子κB(NF-κB)活化以及细胞膜谷胱甘肽过氧化物酶(GSH-PX酶)的影响,以探讨一枝蒿总黄酮的抗乙肝病毒作用机理。方法:采用SABC免疫组织化学染色法检测NF-κB活化的阳性细胞率;采用酶促反应的比色法检测细胞膜GSH-PX酶活性。结果:一枝蒿总黄酮0.250,0.125 mg.mL-1浓度作用后,NF-κB阳性细胞活化率均明显升高,与细胞对照组比较均有显著性差异;一枝蒿总黄酮0.250 mg.mL-1作用后,GSH-PX酶活性明显升高,与细胞对照组比较有显著性差异。结论:一枝蒿总黄酮的体外抗乙肝病毒作用机理与NF-κB活化以及细胞膜的GSH-PX酶活性升高相关。Objective：To study the mechanism of anti-hepatitis B virus by flavonoids extract from milfiol herb,we observed the rate of NF-κB positive cells and activity of GSH-PX on HepG2.2.15 cells in vitro.Method：The rate of NF-κB positive cells and activity of GSH-PX were determined by SABC immunohistochemistry and colorimetry respectively.Result： The rate of NF-κB positive cells increased significantly by treatment of flavonoids extract from milfoil herb at both high and low doses,the activity of GSH-PX increased significantly by treatment of flavonoids extract from milfoil herb at high dose while it increased to some extent for the low dose treatment.Conclusion： The anti-hepatitis B virus effect of flavonoids extract from milfoil herb may be related to increasing of the rate of NF-κB positive cells and the activity of GSH-PX.HepG2.2.15 cells.

关 键 词：一枝蒿总黄酮 乙肝病毒 核转录因子-ΚB 谷胱甘肽过氧化物酶 

分 类 号：R285.5[医药卫生—中药学]